Law Alan Wing Lun, Ahmed Rafay, Cheung Tsz Wing, Mak Chun Yu, Lau Condon
Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region, China.
Biomed Opt Express. 2017 Jan 9;8(2):670-678. doi: 10.1364/BOE.8.000670. eCollection 2017 Feb 1.
We report a novel Raman spectroscopy method for in situ cellular level analysis of the thyroid. Thyroids are harvested from control and lithium treated mice. Lithium is used to treat bipolar disorder, but affects thyroid function. Raman spectra are acquired with a confocal setup (514 nm laser, 20 µm spot) focused on a follicular lumen. Raman peaks are observed at 1440, 1656, and 1746 cm, corresponding to tyrosine, an important amino acid for protein synthesis. Peaks are also observed at 563, 1087, 1265 and 1301 cm. With lithium, the tyrosine peaks increase, indicating tyrosine buildup. Raman spectroscopy can study the impact of many exogenous treatments on thyroid biochemistry.
我们报告了一种用于甲状腺原位细胞水平分析的新型拉曼光谱方法。从对照小鼠和锂处理的小鼠中获取甲状腺。锂用于治疗双相情感障碍,但会影响甲状腺功能。使用聚焦在滤泡腔上的共聚焦装置(514 nm激光,20 µm光斑)采集拉曼光谱。在1440、1656和1746 cm处观察到拉曼峰,对应于酪氨酸,这是蛋白质合成的一种重要氨基酸。在563、1087、1265和1301 cm处也观察到峰。使用锂时,酪氨酸峰增加,表明酪氨酸积累。拉曼光谱可以研究许多外源处理对甲状腺生物化学的影响。