Rillema J A, Linebaugh B E
Horm Metab Res. 1978 Jul;10(4):331-6. doi: 10.1055/s-0028-1093425.
The effects of a variety of agents on guanylate cyclase activity were tested in broken cell preparations of mammary glands from midpregnant mice. Of the agents tested, only phospholipase A, triton X-100, and an impure egg lysolecithin preparation enhanced the activity of guanylate cyclase in mammary gland homogenates; other agents, including sodium azide and phospholipase C, and purified egg lysolecithin had no effect. Phospholipase A increased the activity of guanylate cyclase in the 150,000 g pellet fractions of mammary gland homogenates, bud did not consistently enhance guanylate cyclase in the 150,000 g supernatant fractions. Phospholipase A did not appear to enhance guanylate cyclase activity by solublizing the enzyme from the 150,000 g pellet. Triton X-100, in contrast, appeared to act by solubilizing guanylate cyclase from the material present in the 150,000 g pellet. Triton X-100 increased by several fold guanylate cyclase activity in the tissue homogenates and the 150,000 g pellets, but did not consistently enhance enzyme activity in the 150,000 g supernatant. Triton X-100 had no effect on the apparent Km of guanylate cyclase.
在妊娠中期小鼠乳腺的破碎细胞制剂中测试了多种试剂对鸟苷酸环化酶活性的影响。在所测试的试剂中，只有磷脂酶A、曲拉通X-100和一种不纯的鸡蛋溶血卵磷脂制剂能增强乳腺匀浆中鸟苷酸环化酶的活性；其他试剂，包括叠氮化钠和磷脂酶C，以及纯化的鸡蛋溶血卵磷脂则没有作用。磷脂酶A增加了乳腺匀浆150,000g沉淀组分中鸟苷酸环化酶的活性，但在150,000g上清组分中并不能持续增强鸟苷酸环化酶的活性。磷脂酶A似乎不是通过从150,000g沉淀中溶解酶来增强鸟苷酸环化酶活性的。相比之下，曲拉通X-100似乎是通过从150,000g沉淀中存在的物质中溶解鸟苷酸环化酶来起作用的。曲拉通X-100使组织匀浆和150,000g沉淀中的鸟苷酸环化酶活性增加了几倍，但在150,000g上清中并不能持续增强酶活性。曲拉通X-100对鸟苷酸环化酶的表观Km没有影响。
