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致癌物处理大鼠中结肠隐窝裂变异常

Corrupted colonic crypt fission in carcinogen-treated rats.

作者信息

Rubio Carlos A

机构信息

Department of Pathology, Karolinska Institute and University Hospital, Stockholm, Sweden.

出版信息

PLoS One. 2017 Mar 8;12(3):e0172824. doi: 10.1371/journal.pone.0172824. eCollection 2017.

DOI:10.1371/journal.pone.0172824
PMID:28273142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5342188/
Abstract

BACKGROUND

The colonic crypts in rats reproduce themselves by symmetric fission at the base of the crypts, and proceeding upwards, generate two separate identical crypts. Recently we reported corrupted colonic crypt fission (CCCF) in rats with colonic carcinoma. Here we investigated whether CCCF also occurred in the colonic mucosa without carcinoma in carcinogen-treated rats.

METHODS

Filed Swiss-roll sections from 35 male rats (25 treated with 1,2-dimethyhydrazine (DMH) suspended in EDTA solution, and 10 EDTA-treated) were reviewed. CCCF were regarded those with either asymmetric basal fission, asymmetric lateral sprouting/lateral fission, basal dilatations, or spatial aberrations of the normal (vertical) axis.

RESULTS

202 CCCF (38%) were recorded amongst 533 crypts with fission in DMH-treated rats, and only one CCCF (0.1%) was found amongst 571 crypts with fission in EDTA-treated rats (p<0.05). The basal aspect of four adenomas included in Swiss roll sections exhibited CCCF lined either with indigenous (non-dysplastic) epithelium or with dysplastic epithelium.

CONCLUSION

It was demonstrated that CCCF without dysplasia develop in carcinogen-treated SD rats. As judged by the figures presented, the possibility that the epithelium in those corrupted crypts was successively replaced by top-down growing dysplastic cells, could not be totally rejected. This is the first report showing that non-dysplastic CCCF may antedate the very early stages of colonic carcinogenesis in SD rats.

摘要

背景

大鼠结肠隐窝通过隐窝底部的对称裂变进行自我复制，向上延伸，产生两个独立的相同隐窝。最近我们报道了结肠癌大鼠中存在结肠隐窝裂变异常（CCCF）。在此，我们研究了在致癌物处理的大鼠中，无癌的结肠黏膜中是否也发生CCCF。

方法

回顾了35只雄性大鼠（25只接受溶解于乙二胺四乙酸（EDTA）溶液中的1,2 - 二甲基肼（DMH）处理，10只接受EDTA处理）的存档瑞士卷切片。CCCF被定义为具有不对称基底裂变、不对称侧向发芽/侧向裂变、基底扩张或正常（垂直）轴的空间畸变的隐窝。

结果

在DMH处理的大鼠中，533个有裂变的隐窝中有202个CCCF（38%），而在EDTA处理的大鼠中，571个有裂变的隐窝中仅发现1个CCCF（0.1%）（p<0.05）。瑞士卷切片中的四个腺瘤的基底部分表现出CCCF，内衬为原生（非发育异常）上皮或发育异常上皮。

结论

已证明在致癌物处理的SD大鼠中会发生无发育异常的CCCF。从所呈现的数据判断，那些异常隐窝中的上皮细胞被自上而下生长的发育异常细胞相继替代的可能性不能被完全排除。这是首次报道显示非发育异常的CCCF可能先于SD大鼠结肠癌变的极早期阶段出现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/9c5e750e4293/pone.0172824.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/6eb24dec46a9/pone.0172824.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/e4d123f92a4c/pone.0172824.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/77f07d86665a/pone.0172824.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/96d6b4f33cff/pone.0172824.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/9c5e750e4293/pone.0172824.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/6eb24dec46a9/pone.0172824.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/e4d123f92a4c/pone.0172824.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/77f07d86665a/pone.0172824.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/96d6b4f33cff/pone.0172824.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b8e/5342188/9c5e750e4293/pone.0172824.g005.jpg

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本文引用的文献

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Traditional serrated adenomas and serrated carcinomas in carcinogen-treated rats.致癌物处理大鼠中的传统锯齿状腺瘤和锯齿状癌
在常规腺瘤的肿瘤冠下方，正常上皮衬里的结肠隐窝中发现不同的细胞增殖和 p53 过表达。
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