Rubio C A
Gastrointestinal and Liver Pathology Research Lab, Department of Pathology, Karolinska Institute, 171 76 Stockholm, Sweden.
Apoptosis. 1998;3(1):35-9. doi: 10.1023/a:1009607102797.
The presence of apoptotic bodies and of intraepithelial lymphocytes (IELs) were assessed in colorectal adenomas and adenocarcinomas induced in 158 rats by two different carcinogens: 1,2 dimethylhydrazine (DMH) and glutamic acid pyrolysate (GLU-P-1 and 2). Apoptotic granules were present in 97.5% (n=40) of the 41 GLU-induced adenomas and adenocarcinomas, but only in 20.5% (n=24) of the 117 DMH-induced tumours. IELs were found in 95.1% (n=39) of the 41 GLU-induced tumours but only in 21.4% (n=25) of the 117 DMH-induced neoplasias. The differences were significant (p< 0.001). The presence of IELs and apoptotic granules in GLU tumours (and their absence in the majority of the DMH tumours) is new evidence that IELs are the cells from which many of the apoptotic granules--seen in colorectal neoplasias--derive. GLU neoplasias were induced following daily treatment, for 24 months (about half the life span of the animals) and DMH neoplasias by weekly doses, for a period of only 2.8-6 months. It would appear that 'slowly growing' colorectal GLU neoplasias often attract IELs and trigger lymphocytic apoptosis whereas 'quickly growing' DMH tumours seldom evoke those reactions.
在158只大鼠中，通过两种不同的致癌物（1,2 - 二甲基肼（DMH）和谷氨酸裂解物（GLU - P - 1及GLU - P - 2））诱导产生结直肠腺瘤和腺癌，评估其中凋亡小体和上皮内淋巴细胞（IELs）的存在情况。在41个GLU诱导的腺瘤和腺癌中，97.5%（n = 40）存在凋亡颗粒，但在117个DMH诱导的肿瘤中，仅20.5%（n = 24）存在凋亡颗粒。在41个GLU诱导的肿瘤中，95.1%（n = 39）发现有IELs，但在117个DMH诱导的肿瘤中，仅21.4%（n = 25）发现有IELs。差异具有显著性（p < 0.001）。GLU肿瘤中存在IELs和凋亡颗粒（而大多数DMH肿瘤中不存在）是新的证据，表明IELs是结直肠肿瘤中许多所见凋亡颗粒的来源细胞。GLU肿瘤是在每日给药24个月（约为动物寿命的一半）后诱导产生的，而DMH肿瘤是通过每周给药，为期仅2.8 - 6个月诱导产生的。似乎“生长缓慢”的结直肠GLU肿瘤常吸引IELs并引发淋巴细胞凋亡，而“生长迅速”的DMH肿瘤很少引发这些反应。
