Kim Eun Ji, Cha Mi Na, Kim Bog-Gyu, Ahn Joong-Hoon
Department of Integrative Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea.
Department of Forest Resources, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea.
J Microbiol Biotechnol. 2017 May 28;27(5):975-982. doi: 10.4014/jmb.1701.01030.
Curcumin, a hydrophobic polyphenol derived from the rhizome of the herb , possesses diverse pharmacological properties, including anti-inflammatory, antioxidant, antiproliferative, and antiangiogenic activities. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) were synthesized from glucose in . (phenylalanine ammonia lyase) or (tyrosine ammonia lyase), along with (-coumaroyl-CoA ligase) and (curcumin synthase) genes, were introduced into , and each strain produced dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in , the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, was engineered. Using the engineered strains, the production of bisdemethoxycurcumin increased from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l.
姜黄素是一种从草本植物根茎中提取的疏水性多酚，具有多种药理特性，包括抗炎、抗氧化、抗增殖和抗血管生成活性。在……中，由葡萄糖合成了两种姜黄素类化合物（双肉桂酰甲烷和双去甲氧基姜黄素）。将苯丙氨酸解氨酶（PAL）基因或酪氨酸解氨酶（TAL）基因，以及4-香豆酰辅酶A连接酶（4CL）基因和姜黄素合酶（CURS）基因导入……，每个菌株分别产生双肉桂酰甲烷或双去甲氧基姜黄素。为了提高……中姜黄素类化合物的产量，对莽草酸生物合成途径进行了改造，该途径增加了姜黄素类化合物生物合成的底物。使用工程菌株，双去甲氧基姜黄素的产量从0.32毫克/升增加到4.63毫克/升，双肉桂酰甲烷的产量从1.24毫克/升增加到6.95毫克/升。
