Wang Xiao-Jie, Zheng Xi-Qun, Liu Xiao-Lan, Kopparapu Narasimha-Kumar, Cong Wan-Suo, Deng Yong-Ping
Heilongjiang Provincial Key University Laboratory of Processing Agricultural Products, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, PR China.
Heilongjiang Provincial Key University Laboratory of Processing Agricultural Products, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, PR China.
Food Chem. 2017 Jul 15;227:335-341. doi: 10.1016/j.foodchem.2017.01.069. Epub 2017 Jan 16.
The focus of the present work was to investigate the glycosylation of zein, partial properties of the glycosylated zein (GZ) and its retarding effect on lipid oxidation of ground pork. Zein was glycosylated with chitosan (MW 1500Da) by microbial transglutaminase, the reaction was verified by FT-IR. Under the optimized conditions, 97.48mg of glucosamine was covalently conjugated to 1g of zein, determined by HPLC. The solubility and the surface hydrophobicity of GZ were significantly improved. In vitro studies of GZ showed a dose-dependent scavenging activity against free radicals of DPPH, superoxide and hydroxyl radical, and the EC value for DPPH radical was 1.99μg TE/mg protein. In addition, reducing power and Fe-chelating capacity of it were 16.60 and 12.96μg TE/mg protein, respectively. GZ resulted in low levels of thiobarbituric acid-reactive substances and peroxide value of ground pork. These results suggest that GZ is a potential natural antioxidant.
本研究的重点是研究玉米醇溶蛋白的糖基化、糖基化玉米醇溶蛋白（GZ）的部分性质及其对绞碎猪肉脂质氧化的抑制作用。通过微生物转谷氨酰胺酶使玉米醇溶蛋白与壳聚糖（分子量1500Da）进行糖基化反应，该反应通过傅里叶变换红外光谱（FT-IR）进行验证。在优化条件下，通过高效液相色谱（HPLC）测定，1g玉米醇溶蛋白共价结合了97.48mg氨基葡萄糖。GZ的溶解度和表面疏水性显著提高。GZ的体外研究表明，其对二苯基苦味酰基自由基（DPPH）、超氧阴离子自由基和羟基自由基具有剂量依赖性的清除活性，DPPH自由基的半数有效浓度（EC）值为1.99μg TE/mg蛋白质。此外，其还原力和铁螯合能力分别为16.60和12.96μg TE/mg蛋白质。GZ使绞碎猪肉中的硫代巴比妥酸反应性物质和过氧化值水平降低。这些结果表明，GZ是一种潜在的天然抗氧化剂。
