Ghodrati-Jaldbakhan Shahrbanoo, Ahmadalipour Ali, Rashidy-Pour Ali, Vafaei Abbas Ali, Miladi-Gorji Hossein, Alizadeh Maryam
Student Research Committee, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; Laboratory of Learning and Memory, Research Center and Department of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Student Research Committee, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; Psychiatry and Behavioral Sciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Brain Res. 2017 May 15;1663:20-28. doi: 10.1016/j.brainres.2017.02.024. Epub 2017 Mar 6.
Previous studies from our laboratory have shown that treadmill exercise alleviates the deficits in cognitive functions and anxiety behaviors induced by chronic exposure to morphine in male rats. In this study, we investigated the effects of low and high intensities of treadmill exercise on spatial memory, anxiety-like behaviors, and biochemical changes in the hippocampus and serum of morphine-treated female rats. The adult virgin female rats were injected with bi-daily doses (10mg/kg, at 12h intervals) of morphine over a period of 10days. Following these injections, the rats were exercised under low or high intensities for 30min per session on five days a week for four weeks. After exercise training, object location memory, anxiety profile, hippocampal BDNF, and serum corticosterone and BDNF were examined. Morphine-treated animals exhibited increased anxiety levels, impaired object location memory, and reduced hippocampal BDNF. Exercise alleviated these impairing effects on anxiety profile and memory but not hippocampal BDNF. The high-intensity exercise even further reduced the hippocampal BDNF. Additionally, both exercise regimens in the morphine group and the high exercise in the saline group reduced serum BDNF. Finally, the high-intensity exercise enhanced corticosterone serum. These findings indicate that the negative cognitive and behavioral effects of chronic exposure to morphine could be relieved by forced exercise in female rats. However, the exercise intensity is an important factor to be considered during exercise training. Finally, the correlation between changes of brain and serum BDNF and cognitive functions following morphine exposure needs further research.
我们实验室之前的研究表明，跑步机运动可减轻雄性大鼠长期接触吗啡所导致的认知功能缺陷和焦虑行为。在本研究中，我们调查了低强度和高强度跑步机运动对吗啡处理的雌性大鼠的空间记忆、焦虑样行为以及海马体和血清生化变化的影响。成年未孕雌性大鼠在10天内每隔12小时注射双日剂量（10mg/kg）的吗啡。注射这些药物后，大鼠每周五天、每天进行30分钟的低强度或高强度运动，持续四周。运动训练后，检测物体定位记忆、焦虑状况、海马体脑源性神经营养因子（BDNF）以及血清皮质酮和BDNF。经吗啡处理的动物表现出焦虑水平增加、物体定位记忆受损以及海马体BDNF减少。运动减轻了对焦虑状况和记忆的这些损害作用，但对海马体BDNF没有影响。高强度运动甚至进一步降低了海马体BDNF。此外，吗啡组的两种运动方案以及生理盐水组的高强度运动均降低了血清BDNF。最后，高强度运动提高了血清皮质酮水平。这些发现表明，长期接触吗啡对雌性大鼠产生的负面认知和行为影响可通过强迫运动得到缓解。然而，运动强度是运动训练过程中需要考虑的一个重要因素。最后，吗啡暴露后大脑和血清BDNF变化与认知功能之间的相关性需要进一步研究。
