Liu Xiling, Chen Fang, Niu Yong, Bian Yingnan, Zhang Suhua, Zhu Ruxin, Li Chengtao
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China.
The Judicial Authentication Center of Zhejiang HanBo, Zhejiang 310000, PR China.
Forensic Sci Int Genet. 2017 May;28:e33-e35. doi: 10.1016/j.fsigen.2017.02.010. Epub 2017 Feb 22.
Insertion/deletion (InDels) markers can serve as a useful supporting tool to short tandem repeat (STR) typing systems for human identification. The Qiagen DIPplex Investigator kit, which contains 30 biallelic autosomal InDels and amelogenin, has been developed for forensic use. To estimate the genetic diversity of the 30 markers in Han Chinese individuals living in Zhejiang and to further evaluate their applicability in forensic science, 246 unrelated Han Chinese from Zhejiang were genotyped at these loci. No significant departures from Hardy-Weinberg equilibrium were observed at these loci in these participants. The combined power of discrimination was over 0.99999999 and the combined probability of exclusion was over 0.9901. Results demonstrated that the 30 InDel markers could be used as a supporting tool for the human identification of specific Han Chinese individuals from Zhejiang. The genetic differences and phylogenetic relationships among Han Chinese from Zhejiang, Han Chinese from five other areas, nine minority ethnic groups, as well as two other East Asian populations were also investigated. Two InDel markers, HLD39 and HLD40, showed significant allele-frequency differences between Han Chinese from Zhejiang and ethnic minorities. Further analysis can be used to evaluate their role in forensic science.
插入/缺失（InDels）标记可作为人类身份识别的短串联重复序列（STR）分型系统的有用辅助工具。Qiagen DIPplex Investigator试剂盒包含30个双等位基因常染色体InDels和牙釉蛋白，已开发用于法医鉴定。为了估计浙江汉族人群中这30个标记的遗传多样性，并进一步评估它们在法医学中的适用性，对246名来自浙江的无血缘关系的汉族个体进行了这些位点的基因分型。在这些参与者中，这些位点未观察到显著偏离哈迪-温伯格平衡的情况。联合鉴别力超过0.99999999，联合排除概率超过0.9901。结果表明，这30个InDel标记可作为浙江特定汉族个体身份识别的辅助工具。还研究了浙江汉族与其他五个地区的汉族、九个少数民族以及另外两个东亚人群之间的遗传差异和系统发育关系。两个InDel标记HLD39和HLD40在浙江汉族与少数民族之间显示出显著的等位基因频率差异。进一步分析可用于评估它们在法医学中的作用。
