一种用于法医鉴定的多重插入缺失分析方法在四个中国人群中的验证研究。
A validation study of a multiplex INDEL assay for forensic use in four Chinese populations.
作者信息
Wei Yi-Liang, Qin Cui-Jiao, Dong Hui, Jia Jing, Li Cai-Xia
机构信息
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China; Key Laboratory of Medical Epigenetics, Tianjin Research Center of Basic Medical Sciences, Tianjin 300070, PR China.
Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, PR China; Department of Forensic Science, Chinese People's Public Security University, Beijing 100038, PR China.
出版信息
Forensic Sci Int Genet. 2014 Mar;9:e22-5. doi: 10.1016/j.fsigen.2013.09.002. Epub 2013 Sep 17.
Insertion/deletion (INDELs) marker sets can serve as a useful supplementary tool for human identification. A commercial kit, the Qiagen DIPplex(®) Investigator kit, multiplexes 30 biallelic autosomal INDELs and Amelogenin for forensic use. We performed a validation study based on the DIPplex(®) kit in four Chinese populations: Han, Tibetan, Uyghur, and Kazakh. There were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium (pair-wised r(2)<0.2) between the 30 INDELs. The random match probabilities were in the range of 3.84 × 10(-11) to 1.20 × 10(-12), and the power of exclusion was >0.99. The multiplex PCR was optimized for a 5-μL volume, full profiles were obtained with 0.062 ng/μL of template DNA, and excellent performance was obtained with degraded casework samples. This study demonstrates that the multiplex INDEL assay can be used as a supplementary method for degraded DNA detection in the studied Chinese populations.
插入/缺失（INDELs）标记集可作为人类身份识别的有用补充工具。一种商业试剂盒，即Qiagen DIPplex® Investigator试剂盒，可对30个双等位基因常染色体INDELs和牙釉蛋白基因进行多重检测，用于法医鉴定。我们基于DIPplex®试剂盒在四个中国人群（汉族、藏族、维吾尔族和哈萨克族）中进行了验证研究。这30个INDELs之间没有显著偏离哈迪-温伯格平衡，也没有显著的连锁不平衡（成对r²<0.2）。随机匹配概率在3.84×10⁻¹¹至1.20×10⁻¹²之间，排除概率>0.99。多重PCR针对5μL体积进行了优化，使用0.062 ng/μL的模板DNA可获得完整图谱，对降解的实际案件样本也有出色表现。本研究表明，多重INDEL检测可作为在所研究的中国人群中检测降解DNA的补充方法。
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