Romero R, Quintero R, Emamian M, Wan M, Grzyboski C, Hobbins J C, Mitchell M D
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
Am J Obstet Gynecol. 1987 Dec;157(6):1454-60. doi: 10.1016/s0002-9378(87)80243-0.
This study was undertaken to examine the effects of intrauterine infection and preterm labor on the amniotic fluid concentrations of arachidonate lipoxygenase metabolites in women with premature rupture of membranes. Amniotic fluid was obtained from four groups of women with premature rupture of membranes: group 1, women without labor or infection; group 2, women with labor but without infection; group 3, women with intra-amniotic infection but without labor; and group 4, women with both infection and labor. 12-Hydroxyeicosatetraenoic acid, 15-hydroxyeicosatetraenoic acid, and leukotriene B4 were measured by radioimmunoassays. Amniotic fluid concentrations of 12-hydroxyeicosatetraenoic acid were found not to differ significantly among the four groups. Amniotic fluid concentrations of 15-hydroxyeicosatetraenoic acid in group 4 were significantly higher than in women in groups 1 and 3 (p less than 0.05). In addition, amniotic fluid concentrations in leukotriene B4 were significantly higher in group 4 than in any of the other three groups (p less than 0.05). Leukotriene B4 concentrations were higher in groups 2 and 3 than in group 1, suggesting that the presence of both labor and infection increases the concentration of this metabolite in amniotic fluid. Infection and labor had an additive effect in the elevation of amniotic fluid concentrations of leukotriene B4. These results suggest that the amniotic fluid concentrations of arachidonate lipoxygenase metabolites are affected differently by the presence of infection and labor in women with premature rupture of membranes.
本研究旨在探讨宫内感染和早产对胎膜早破女性羊水中花生四烯酸脂氧合酶代谢产物浓度的影响。从四组胎膜早破女性中获取羊水：第1组，未临产或未感染的女性；第2组，临产但未感染的女性；第3组，羊膜腔内感染但未临产的女性；第4组，既感染又临产的女性。通过放射免疫分析法测定12-羟基二十碳四烯酸、15-羟基二十碳四烯酸和白三烯B4。发现四组之间12-羟基二十碳四烯酸的羊水浓度无显著差异。第4组中15-羟基二十碳四烯酸的羊水浓度显著高于第1组和第3组女性（p<0.05）。此外，第4组中白三烯B4的羊水浓度显著高于其他三组中的任何一组（p<0.05）。第2组和第3组中白三烯B4的浓度高于第1组，表明临产和感染均会增加羊水中该代谢产物的浓度。感染和临产在升高羊水中白三烯B4浓度方面具有相加作用。这些结果表明，胎膜早破女性中，感染和临产的存在对花生四烯酸脂氧合酶代谢产物的羊水浓度有不同影响。
