Berger Annemarie, Salla Sabine, Keppler Oliver T, Rabenau Holger F
Institute of Medical Virology, Hospital of the Goethe University of Frankfurt, Frankfurt/M., Germany.
Department of Ophthalmology, RWTH Aachen University, Germany.
Transfus Med Hemother. 2017 Jan;44(1):39-44. doi: 10.1159/000449207. Epub 2016 Nov 4.
The HCV RNA testing of potential cornea donors frequently relies on blood samples stored pre mortem. The recommended storage time of maximum 72 h frequently excludes a significant fraction of donors.
The influence of storage time of EDTA plasma samples at 4 °C on the viral load measured with the Roche HCV Quantitative Test vs. 2.0 was evaluated for 43 samples from HCV-positive individuals.
The mean reduction of the viral load after 4 °C storage for 6-8 days was 0.46 log IU/ml (range +0.17 to -1.66 log IU/ml). After 1-3 days a mean loss of 0.19 log IU/ml (range +0.30 to -1.41 log IU/ml) and after 3-5 days of 0.32 log IU/ml (range +0.36 to -1.81 log IU/ml) was observed. In 23.3% of samples, a viral load reduction ≥ 1 log IU/ml (1.0-1.81 log IU/ml) was found after prolonged storage (5-8 days). In none of the samples did the HCV load fall below the detection limit.
Plasma storage for up to 8 days can quantitatively reduce the HCV RNA load, yet has no influence on the reliability of a qualitative HCV RNA detection by this ultrasensitive test to determine the HCV status of serologically negative cornea donors.
潜在角膜供体的丙型肝炎病毒（HCV）RNA检测通常依赖于生前采集并储存的血液样本。建议的最长储存时间为72小时，这常常将很大一部分供体排除在外。
对43份来自HCV阳性个体的样本，评估了在4℃条件下EDTA血浆样本的储存时间对采用罗氏HCV定量检测2.0版所测病毒载量的影响。
在4℃储存6 - 8天后，病毒载量平均下降0.46 log IU/ml（范围为+0.17至 - 1.66 log IU/ml）。在储存1 - 3天后，平均下降0.19 log IU/ml（范围为+0.30至 - 1.41 log IU/ml），在储存3 - 5天后，平均下降0.32 log IU/ml（范围为+0.36至 - 1.81 log IU/ml）。在23.3%的样本中，长时间储存（5 - 8天）后病毒载量下降≥1 log IU/ml（1.0 - 1.81 log IU/ml）。没有一个样本的HCV载量降至检测限以下。
血浆储存长达8天可定量降低HCV RNA载量，但对于通过这种超灵敏检测来定性检测HCV RNA以确定血清学阴性角膜供体的HCV状态的可靠性没有影响。
