TaqMan 实时 PCR 法检测血样中丙型肝炎病毒 RNA 的稳定性。
Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR.
机构信息
Department of Medical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey.
出版信息
J Clin Lab Anal. 2010;24(3):134-8. doi: 10.1002/jcla.20354.
The storage conditions of blood samples for reliable results are very important in hepatitis C virus (HCV) RNA amplification tests used in routine HCV analyses. According to many studies, storage conditions could affect the RNA stability for HCV RNA detection. We have studied HCV RNA stability in blood samples stored at 4 degrees C. Nineteen blood samples containing different HCV RNA levels were stored at 4 degrees C and they were then analyzed by TaqMAN real-time PCR method. HCV RNA levels remained almost stable (100%) at least for five weeks at this storage condition. However, among them, the stability period was up to 11 weeks in two of the samples. As with these findings, there was a slightly significant correlation between the positivity time and the beginning HCV RNA levels (r=0.474, P=0.040). We conclude that, blood samples can be stored at 4 degrees C for five weeks without any significant difference in detected HCV RNA level by using TaqMan real-time PCR.
在丙型肝炎病毒 (HCV) RNA 扩增检测中，为了获得可靠的结果，血液样本的储存条件非常重要，这些检测常用于常规 HCV 分析。根据多项研究，储存条件可能会影响 HCV RNA 检测的稳定性。我们研究了在 4°C 下储存的血液样本中 HCV RNA 的稳定性。将 19 份含有不同 HCV RNA 水平的血液样本储存在 4°C 下，然后通过 TaqMAN 实时 PCR 方法进行分析。在这种储存条件下，HCV RNA 水平至少在五周内保持几乎稳定（100%）。然而，其中两份样本的稳定期长达 11 周。与这些发现一致的是，阳性时间与 HCV RNA 水平的起始值之间存在略微显著的相关性（r=0.474，P=0.040）。我们的结论是，使用 TaqMan 实时 PCR 检测时，血液样本可以在 4°C 下储存五周，而 HCV RNA 水平没有明显差异。
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