• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Ca2+ regulation of 1-(3-sn-phosphatidyl)-1D-myo-inositol 4-phosphate formation and hydrolysis on sarcoplasmic-reticular Ca2+-transport ATPase. A new principle of phospholipid turnover regulation.肌浆网Ca2+转运ATP酶上Ca2+对1-(3-sn-磷脂酰)-1D-肌醇4-磷酸形成与水解的调节。磷脂周转调节的新原理。
Biochem J. 1987 Nov 1;247(3):579-87. doi: 10.1042/bj2470579.
2
Influence of Ca2+ and Mg2+ on the turnover of the phosphomonoester group of phosphatidylinositol 4-phosphate in human erythrocyte membranes.钙离子和镁离子对人红细胞膜中磷脂酰肌醇4-磷酸磷酸单酯基团周转的影响。
Biochem J. 1987 May 15;244(1):183-90. doi: 10.1042/bj2440183.
3
Polyphosphoinositide formation in isolated cardiac plasma membranes.分离的心肌细胞质膜中多磷酸肌醇的形成
Lipids. 1989 Sep;24(9):818-23. doi: 10.1007/BF02544590.
4
Activation of sarcoplasmic reticular Ca2+ transport ATPase by phosphorylation of an associated phosphatidylinositol.通过相关磷脂酰肌醇的磷酸化激活肌浆网Ca2+转运ATP酶
EMBO J. 1983;2(9):1543-8. doi: 10.1002/j.1460-2075.1983.tb01621.x.
5
Effects of carbachol and pancreozymin (cholecystokinin-octapeptide) on polyphosphoinositide metabolism in the rat pancreas in vitro.体外实验中卡巴胆碱和促胰液素（胆囊收缩素八肽）对大鼠胰腺多磷酸肌醇代谢的影响
Biochem J. 1984 Jan 1;217(1):281-7. doi: 10.1042/bj2170281.
6
Stimulation of phosphatidylinositol phosphorylation in the sarcoplasmic reticular Ca2+-transport ATPase by vanadate.钒酸盐对肌浆网Ca2+转运ATP酶中磷脂酰肌醇磷酸化的刺激作用。
Z Naturforsch C J Biosci. 1986 Mar;41(3):310-4. doi: 10.1515/znc-1986-0311.
7
Rapid breakdown of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in rat hepatocytes stimulated by vasopressin and other Ca2+-mobilizing hormones.血管加压素和其他可动员钙离子的激素刺激下大鼠肝细胞中磷脂酰肌醇4-磷酸和磷脂酰肌醇4,5-二磷酸的快速分解。
Biochem J. 1983 Jun 15;212(3):733-47. doi: 10.1042/bj2120733.
8
Phosphoinositide hydrolysis by guanosine 5'-[gamma-thio]triphosphate-activated phospholipase C of turkey erythrocyte membranes.火鸡红细胞膜中由鸟苷5'-[γ-硫代]三磷酸激活的磷脂酶C引起的磷酸肌醇水解作用。
Biochem J. 1988 Jun 1;252(2):583-93. doi: 10.1042/bj2520583.
9
The hydrolysis of phosphatidylinositol 4-phosphate in membranes of Xenopus laevis oocytes: characteristics of a phosphomonoesterase.
Comp Biochem Physiol B. 1991;100(4):809-16. doi: 10.1016/0305-0491(91)90295-o.
10
Reconstitution of the skeletal sarcoplasmic reticulum Ca2(+)-pump: influence of negatively charged phospholipids.骨骼肌肌浆网Ca2+泵的重组：带负电荷磷脂的影响
Biochim Biophys Acta. 1991 Jan 31;1091(2):127-34. doi: 10.1016/0167-4889(91)90051-x.

引用本文的文献

1
Neomycin inhibits the phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate stimulation of plasma membrane ATPase activity.新霉素抑制磷脂酰肌醇单磷酸和磷脂酰肌醇二磷酸对质膜ATP酶活性的刺激作用。
Plant Physiol. 1991 May;96(1):340-3. doi: 10.1104/pp.96.1.340.
2
Purification and Characterization of a Soluble Phosphatidylinositol 4-Kinase from Carrot Suspension Culture Cells.胡萝卜悬浮培养细胞中可溶性磷脂酰肌醇4-激酶的纯化与特性分析
Plant Physiol. 1995 Feb;107(2):491-500. doi: 10.1104/pp.107.2.491.
3
Anionic phospholipids decrease the rate of slippage on the Ca(2+)-ATPase of sarcoplasmic reticulum.阴离子磷脂降低了肌浆网Ca(2+)-ATP酶上的滑动速率。
Biochem J. 1999 Sep 1;342 ( Pt 2)(Pt 2):431-8.
4
Uncoupling of Ca2+ transport ATPase in muscle and blood platelets by diacylglycerol analogues and cyclosporin A antagonism.二酰基甘油类似物和环孢菌素A拮抗作用对肌肉和血小板中Ca2+转运ATP酶的解偶联作用。
Biochem J. 1997 Nov 1;327 ( Pt 3)(Pt 3):795-801. doi: 10.1042/bj3270795.
5
Changes in phosphatidylinositol metabolism in response to hyperosmotic stress in Daucus carota L. cells grown in suspension culture.悬浮培养的胡萝卜（Daucus carota L.）细胞中磷脂酰肌醇代谢对高渗胁迫的响应变化
Plant Physiol. 1993 Oct;103(2):637-47. doi: 10.1104/pp.103.2.637.
6
Polyphosphoinositide formation in isolated cardiac plasma membranes.分离的心肌细胞质膜中多磷酸肌醇的形成
Lipids. 1989 Sep;24(9):818-23. doi: 10.1007/BF02544590.
7
The plant phosphoinositide system.植物磷酸肌醇系统。
Biochem J. 1992 Dec 15;288 ( Pt 3)(Pt 3):697-712. doi: 10.1042/bj2880697.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
3
Activation of sarcoplasmic reticular Ca2+ transport ATPase by phosphorylation of an associated phosphatidylinositol.通过相关磷脂酰肌醇的磷酸化激活肌浆网Ca2+转运ATP酶
EMBO J. 1983;2(9):1543-8. doi: 10.1002/j.1460-2075.1983.tb01621.x.
4
Phosphorylation of phosphatidylinositol in rat liver Golgi.大鼠肝脏高尔基体中磷脂酰肌醇的磷酸化作用
J Biol Chem. 1983 Jul 10;258(13):7968-73.
5
Phosphorylation of isocitrate dehydrogenase as a demonstration of enhanced sensitivity in covalent regulation.异柠檬酸脱氢酶的磷酸化作为共价调节中敏感性增强的一种表现。
Nature. 1983;305(5932):286-90. doi: 10.1038/305286a0.
6
Phosphorylation of rat liver nuclear envelopes. II. Characterization of in vitro lipid phosphorylation.大鼠肝细胞核被膜的磷酸化作用。II. 体外脂质磷酸化的特性
J Biol Chem. 1983 Aug 10;258(15):9368-73.
7
Identification of phosphatidylinositol kinase in rat liver lysosomal membranes.大鼠肝脏溶酶体膜中磷脂酰肌醇激酶的鉴定
J Biol Chem. 1983 Feb 25;258(4):2130-4.
8
Extraction and purification of polyphosphoinositides.多磷酸肌醇的提取与纯化。
Methods Enzymol. 1981;72:626-31. doi: 10.1016/s0076-6879(81)72054-8.
9
Phase separation of integral membrane proteins in Triton X-114 solution.整合膜蛋白在Triton X-114溶液中的相分离。
J Biol Chem. 1981 Feb 25;256(4):1604-7.
10
Ca2+-Mg2+-ATPase in rat hepatocyte plasma membranes: inhibition by vasopressin and purification of the enzyme.
Prog Clin Biol Res. 1984;168:25-30.

肌浆网Ca2+转运ATP酶上Ca2+对1-(3-sn-磷脂酰)-1D-肌醇4-磷酸形成与水解的调节。磷脂周转调节的新原理。

Ca2+ regulation of 1-(3-sn-phosphatidyl)-1D-myo-inositol 4-phosphate formation and hydrolysis on sarcoplasmic-reticular Ca2+-transport ATPase. A new principle of phospholipid turnover regulation.

作者信息

Schäfer M, Behle G, Varsányi M, Heilmeyer L M

机构信息

Lehrstuhl für Physiologische Chemie, Ruhr-Universität Bochum, Federal Republic of Germany.

出版信息

Biochem J. 1987 Nov 1;247(3):579-87. doi: 10.1042/bj2470579.

DOI:10.1042/bj2470579
PMID:2827632
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148452/
Abstract

Lipid phosphorylation was shown to occur on the isolated sarcoplasmic-reticulum (SR) Ca2+-transport ATPase. More than 95% of the radioactivity incorporated on incubation of the SR ATPase with [gamma-32P]ATPMg can be extracted with acidic organic solvents and was identified as 1-(3-sn-phosphatidyl)-1D-myo-inositol 4-phosphate (PtdIns4P) [Varsányi, Toelle, Heilmeyer, Dawson & Irvine (1983) EMBO J. 2, 1543-1548]. This lipid phosphorylation is only observed at nanomolar concentrations of free Ca2+; in the presence of micromolar free Ca2+ PtdIns4P disintegrates rapidly. Also, upon blockade of the kinase reaction PtdIns4P decomposes, indicating a PtdIns/PtdIns4P turnover. The PtdIns4P concentration is dependent on the free Ca2+ concentration, being half-maximal at 35 nM-Ca2+. PtdIns4P hydrolysis is catalysed by a PtdIns4P phosphomonoesterase; accordingly no diacylglycerol is formed, which would be a product of a phosphodiesteratic cleavage. Fluoride inhibits this phosphomonoesterase. Ca2+ does not influence directly either the PtdIns kinase or the PtdIns4P phosphomonoesterase. PtdIns4P forms a tight complex with the transport ATPase, from which it can be removed only by chromatography on heparin-agarose in the presence of Triton X-100. It is concluded that Ca2+ regulates the PtdIns/PtdIns4P turnover by availability of substrate, depending on the Ca2+-transport-ATPase conformation, which traps or exposes the respective lipid head groups.

摘要

脂质磷酸化已被证明发生在分离的肌浆网（SR）钙转运ATP酶上。在用[γ-32P]ATP镁孵育SR ATP酶时，超过95%掺入的放射性可被酸性有机溶剂提取，并被鉴定为1-(3-sn-磷脂酰)-1D-肌醇4-磷酸（磷脂酰肌醇4磷酸，PtdIns4P）[瓦尔萨尼、特勒、海尔迈尔、道森和欧文（1983年）《欧洲分子生物学组织杂志》2，1543 - 1548]。这种脂质磷酸化仅在纳摩尔浓度的游离钙离子下观察到；在微摩尔浓度的游离钙离子存在时，PtdIns4P会迅速分解。此外，激酶反应被阻断时，PtdIns4P会分解，表明存在磷脂酰肌醇/磷脂酰肌醇4磷酸的周转。PtdIns4P的浓度取决于游离钙离子的浓度，在35 nM - Ca2+时达到半数最大浓度。PtdIns4P水解由一种PtdIns4P磷酸单酯酶催化；因此不会形成二酰基甘油，而二酰基甘油是磷酸二酯酶裂解的产物。氟化物会抑制这种磷酸单酯酶。钙离子不会直接影响磷脂酰肌醇激酶或PtdIns4P磷酸单酯酶。PtdIns4P与转运ATP酶形成紧密复合物，只有在Triton X - 100存在的情况下通过肝素琼脂糖柱层析才能将其去除。结论是钙离子通过底物的可用性来调节磷脂酰肌醇/磷脂酰肌醇4磷酸的周转，这取决于钙转运ATP酶的构象，该构象会捕获或暴露相应的脂质头部基团。