损伤角膜中血小板活化因子的积累可能与脂氧合酶产物的合成相互关联。
The accumulation of platelet activating factor in the injured cornea may be interrelated with the synthesis of lipoxygenase products.
作者信息
Bazan H E, Reddy S T, Woodland J M, Bazan N G
机构信息
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112.
出版信息
Biochem Biophys Res Commun. 1987 Dec 31;149(3):915-20. doi: 10.1016/0006-291x(87)90495-5.
The rabbit cornea accumulates platelet activating factor (PAF) three hrs after alkali burn. PAF was isolated by HPLC and assayed by platelet aggregation. This bioactivity was blocked by the PAF receptor antagonists BN 52021 and alprazolam. Added PAF increases the chemiluminescence response of the cornea in vitro and BN 52021 inhibits this effect. In vivo experiments show that the synthesis of 5-HETE and 12-HETE is inhibited by the PAF antagonist BN 52021. It is concluded that a metabolic interrelationship may exist between the PAF cycle and the lipoxygenation of arachidonic acid, and that drugs that affect these lipid mediators may modulate the inflammatory response of the anterior segment of the eye.
兔角膜在碱烧伤后3小时会积累血小板活化因子（PAF）。通过高效液相色谱法（HPLC）分离PAF，并通过血小板聚集进行测定。这种生物活性被PAF受体拮抗剂BN 52021和阿普唑仑阻断。添加的PAF会增加角膜在体外的化学发光反应，而BN 52021会抑制这种作用。体内实验表明，PAF拮抗剂BN 52021可抑制5-羟基二十碳四烯酸（5-HETE）和12-羟基二十碳四烯酸（12-HETE）的合成。得出的结论是，PAF循环与花生四烯酸的脂氧化之间可能存在代谢相互关系，并且影响这些脂质介质的药物可能会调节眼前段的炎症反应。
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