a Department of Periodontology, The First Clinical Division , Peking University School and Hospital of Stomatology , Beijing , China.
b Department of General Dentistry II , Peking University School and Hospital of Stomatology , Beijing , China.
Platelets. 2017 Dec;28(8):774-778. doi: 10.1080/09537104.2016.1267338. Epub 2017 Feb 21.
Concentrated growth factor (CGF), a new generation of platelet concentrate product, appears to have more abundant growth factors because of its special centrifugation process. However, there are few studies supporting this. This study was designed to evaluate the contents of major growth factors in CGF and compare them with those found in PRP (platelet-rich plasma) and PRF (platelet-rich fibrin). PRP, PRF, and CGF were obtained from the same samples of peripheral blood. Concentrations of five representative growth factors in platelets were measured with enzyme-linked immunosorbent assay (ELISA): platelet-derived growth factor-BB (PDGF-BB), transforming growth factor β-1 (TGF-β1), insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF). The results showed that the bFGF levels in CGF and PRF were significantly higher than that in activated PRP. For other growth factors, such as PDGF-BB, TGF-β1, IGF-1, and VEGF, the levels did not differ significantly among activated PRP, PRF, and CGF. Our findings extended the currently available data on the release and measurement of growth factors in CGF and other platelet gels. In future studies, we need more data to find the proper therapeutic doses for platelet concentrates suitable for different clinical applications.
浓缩生长因子（CGF）是一种新一代血小板浓缩产品，由于其特殊的离心过程，似乎具有更丰富的生长因子。然而，支持这一观点的研究很少。本研究旨在评估 CGF 中主要生长因子的含量，并将其与富血小板血浆（PRP）和富血小板纤维蛋白（PRF）中的含量进行比较。PRP、PRF 和 CGF 均从外周血的相同样本中获得。采用酶联免疫吸附试验（ELISA）测定血小板中 5 种代表性生长因子的浓度：血小板衍生生长因子-BB（PDGF-BB）、转化生长因子-β1（TGF-β1）、胰岛素样生长因子-1（IGF-1）、血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）。结果表明，CGF 和 PRF 中的 bFGF 水平明显高于激活的 PRP。对于其他生长因子，如 PDGF-BB、TGF-β1、IGF-1 和 VEGF，激活的 PRP、PRF 和 CGF 之间的水平没有显著差异。我们的研究结果扩展了目前关于 CGF 和其他血小板凝胶中生长因子释放和测量的可用数据。在未来的研究中，我们需要更多的数据来找到适合不同临床应用的血小板浓缩物的合适治疗剂量。
