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果蔬废弃物高固体厌氧单级消化的微生物和营养调控

Microbial and nutritional regulation of high-solids anaerobic mono-digestion of fruit and vegetable wastes.

作者信息

Mu Hui, Li Yan, Zhao Yuxiao, Zhang Xiaodong, Hua Dongliang, Xu Haipeng, Jin Fuqiang

机构信息

a Energy Research Institute of Shandong Academy of Sciences , Key Laboratory for Biomass Gasification Technology of Shandong Province , Jinan , People's Republic of China.

出版信息

Environ Technol. 2018 Feb;39(4):405-413. doi: 10.1080/09593330.2017.1301571. Epub 2017 Mar 16.

DOI:10.1080/09593330.2017.1301571
PMID:28278097
Abstract

The anaerobic digestion of single fruit and vegetable wastes (FVW) can be easily interrupted by rapid acidogenesis and inhibition of methanogen, and the digestion system tends to be particularly unstable at high solid content. In this study, the anaerobic digestion of FVW in batch experiments under mesophilic condition at a high solid concentration of 10% was successfully conducted to overcome the acidogenesis problem through several modifications. Firstly, compared with the conventional anaerobic sludge (CAS), the acclimated anaerobic granular sludge (AGS) was found to be a better inoculum due to its higher Archaea abundance. Secondly, waste activated sludge (WAS) was chosen to co-digest with FVW, because WAS had abundant proteins that could generate intermediate ammonium. The ammonium could neutralize the accumulated volatile fatty acids (VFAs) and prevent the pH value of the digestion system from rapidly decreasing. Co-digestion of FVW and WAS with TS ratio of 60:40 gave the highest biogas yield of 562 mL/g-VS and the highest methane yield of 362 mL/g-VS. Key parameters in the digestion process, including VFAs concentration, pH, enzyme activity, and microbial activity, were also examined.

摘要

单一果蔬废弃物(FVW)的厌氧消化很容易因快速产酸和甲烷菌受到抑制而中断,并且在高固体含量下消化系统往往特别不稳定。在本研究中,通过若干改进措施,成功地在中温条件下、10%的高固体浓度下对FVW进行了批次厌氧消化实验,以克服产酸问题。首先,与传统厌氧污泥(CAS)相比,驯化后的厌氧颗粒污泥(AGS)由于其较高的古菌丰度,被发现是一种更好的接种物。其次,选择剩余活性污泥(WAS)与FVW共同消化,因为WAS含有丰富的蛋白质,能够产生中间产物铵。铵可以中和积累的挥发性脂肪酸(VFA),防止消化系统的pH值迅速下降。FVW与WAS以60:40的总固体(TS)比例共同消化时,沼气产量最高,为562 mL/g-VS,甲烷产量最高,为362 mL/g-VS。还研究了消化过程中的关键参数,包括VFA浓度、pH值、酶活性和微生物活性。

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