Li Shaoli, Geng Jinju, Wu Gang, Gao Xingsheng, Fu Yingying, Ren Hongqiang
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China.
Sci Rep. 2018 Feb 21;8(1):3399. doi: 10.1038/s41598-018-21564-x.
Concern is growing over contamination of the environment with artificial sweeteners (ASWs) because of their widespread existence in wastewater treatment plants (WWTPs). To evaluate ASWs removal and the effect on activated sludge, acesulfame (ACE), sucralose (SUC), cyclamate (CYC) and saccharin (SAC) were introduced individually or in mixture to sequencing batch reactors (SBRs) in environmentally relevant concentrations (100 ppb) for 100 days. Comparisons between ACE removal in a full-scale WWTP and in lab-scale SBRs were conducted. Results showed that CYC and SAC were completely removed, whereas SUC was persistent. However, ACE removal in lab-scale SBRs was significantly greater than in the full-scale WWTP. In SBRs, chemical oxygen demand (COD), ammonia nitrogen (NH-N) and total nitrogen (TN) removal appeared unchanged after adding ASWs (p > 0.05). Adenosine triphosphate (ATP) concentrations and triphenyl tetrazolium chloride-dehydrogenase activity (TTC-DHA) declined significantly (p < 0.05). The mixed ASWs had more evident effects than the individual ASWs. Microbial community analyses revealed that Proteobacteria decreased obviously, while Bacteroidetes, Chloroflexi and Actinobacteria were enriched with the addition of ASWs. Redundancy Analysis (RDA) indicated ACE had a greater impact on activated sludge than the other ASWs.
由于人工甜味剂（ASWs）在污水处理厂（WWTPs）中广泛存在，人们对其对环境污染的担忧日益增加。为了评估ASWs的去除情况及其对活性污泥的影响，将乙酰磺胺酸钾（ACE）、三氯蔗糖（SUC）、甜蜜素（CYC）和糖精（SAC）以环境相关浓度（100 ppb）单独或混合引入序批式反应器（SBRs）中，持续100天。对实际规模污水处理厂和实验室规模SBRs中ACE的去除情况进行了比较。结果表明，CYC和SAC被完全去除，而SUC具有持久性。然而，实验室规模SBRs中ACE的去除率显著高于实际规模的污水处理厂。在SBRs中，添加ASWs后化学需氧量（COD）、氨氮（NH-N）和总氮（TN）的去除率似乎没有变化（p>0.05）。三磷酸腺苷（ATP）浓度和氯化三苯基四氮唑脱氢酶活性（TTC-DHA）显著下降（p<0.05）。混合ASWs的影响比单一ASWs更明显。微生物群落分析表明，添加ASWs后变形菌明显减少，而拟杆菌门、绿弯菌门和放线菌门则有所富集。冗余分析（RDA）表明，ACE对活性污泥的影响比其他ASWs更大。
