Tang Huaqi, Zhang Xinyue, Luo Shilin, Shang Fei, Chen Jianping, Wang Jinghong, Duan Xiaojie, Gao Xiaoyan, Sun Yikun
a School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
b Analytical and Testing Center , Beijing University of Chemical Technology , Beijing , China.
Nat Prod Res. 2017 Jul;31(14):1620-1624. doi: 10.1080/14786419.2017.1285296. Epub 2017 Feb 15.
Xuan Hu Suo San (XHSS) is a traditional Chinese medicine that has been extensively applied in the treatment of osteoarthritis for many years, however, its chemical composition has not yet been elucidated. Thus, a rapid, efficient, and precise method based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was applied in both positive- and negative-ion modes to rapidly separate and identify the main chemical ingredients in XHSS for the first time. Finally, with the optimised separation and detection method, a total of 57 compounds were simultaneously separated within 13 min, among which 14 compounds were confirmed by comparing retention time and MS data with reference standards and others were tentatively identified by comparing with reference literatures. This rapid and sensitive approach is highly useful for the identification and characterisation of chemical constituents, and provides fundamental and extensive information supporting further metabolic and pharmacokinetic studies of XHSS.
玄胡索散（XHSS）是一种多年来广泛应用于治疗骨关节炎的中药，然而，其化学成分尚未阐明。因此，首次采用基于超高效液相色谱-四极杆飞行时间质谱联用的快速、高效、精确方法，在正离子和负离子模式下对玄胡索散中的主要化学成分进行快速分离和鉴定。最后，通过优化的分离和检测方法，在13分钟内同时分离出57种化合物，其中14种化合物通过与参考标准品比较保留时间和质谱数据得到确认，其他化合物通过与参考文献比较进行初步鉴定。这种快速灵敏的方法对于化学成分的鉴定和表征非常有用，并为玄胡索散进一步的代谢和药代动力学研究提供了基础且广泛的信息。
