Qing Zhi-Xing, Yang Peng, Yu Kun, Yang Xue-Yi, Liu Jing-Hong, Xiang Feng, Cao Hua-Liang, Cheng Pi, Zeng Jian-Guo
a College of Pharmacy , Hunan University of Chinese Medicine , Changsha , China.
d Pharmacy of Traditional Medicine , Tongde Hospital of Zhejiang Province , Zhejiang , China.
Nat Prod Res. 2017 Jul;31(14):1633-1639. doi: 10.1080/14786419.2017.1285300. Epub 2017 Feb 8.
Two new alkaloids 6-hydroxyethyldihydrochelerythrine (1) and 6-hydroxymethyl-7,8-demethylenedihydrochelerythrine (2) together with two analogues named maclekarpine E (3) and 6-hydroxymethyldihydrosanguinarine (4) were detected primarily from the leaves of Macleaya cordata by their characteristic mass spectrometry (MS) and tandem mass spectrometry (MS/MS). And then isolation of four targeted-compounds was performed by column chromatography and preparation HPLC under the guiding of MS. Finally, their structures were determined by spectrum analysis. Alkaloids 3 and 4 were isolated from this plant medicine for the first time.
通过特征质谱（MS）和串联质谱（MS/MS），首次从博落回叶片中主要检测到两种新生物碱6-羟基乙基二氢白屈菜红碱（1）和6-羟甲基-7,8-去亚甲基二氢白屈菜红碱（2），以及两种类似物maclekarpine E（3）和6-羟甲基二氢血根碱（4）。然后在质谱引导下，通过柱色谱和制备型高效液相色谱法对四种目标化合物进行分离。最后，通过光谱分析确定其结构。生物碱3和4首次从这种植物药中分离得到。
