Wang Yun, Wang Qian, Wu Ai-Hua, Hao Zhen-Ping, Liu Xian-Jin
College of Horticulture, Jinling Institute of Technology, 210038 Nanjing, PR China.
College of Horticulture, Jinling Institute of Technology, 210038 Nanjing, PR China.
Anal Biochem. 2017 Dec 15;539:29-32. doi: 10.1016/j.ab.2017.03.004. Epub 2017 Mar 6.
Traditional ELISA methods of using animal immunity yield antibodies for detection Cry toxin. Not only is this incredibly harmful to the animals, but is also time-intensive. Here we developed a simple method to yield the recognition element. Using a critical selection strategy and immunoassay we confirmed a clone from the Ph.D-C7C phage library, which has displayed the most interesting Cry1Ab-binding characteristics examined in this study (Fig. 1). The current study indicates that isolating peptide is an alternative method for the preparation of a recognition element, and that the developed assay is a potentially useful tool for detecting Cry1Ab.
传统的利用动物免疫的酶联免疫吸附测定（ELISA）方法可产生用于检测Cry毒素的抗体。这不仅对动物极其有害，而且耗时。在此，我们开发了一种产生识别元件的简单方法。通过关键筛选策略和免疫测定，我们从Ph.D-C7C噬菌体文库中确认了一个克隆，该克隆在本研究中表现出最有趣的Cry1Ab结合特性（图1）。当前研究表明，分离肽是制备识别元件的一种替代方法，并且所开发的测定法是检测Cry1Ab的一种潜在有用工具。
