Dai Zao, Li Jicheng, Hu Chengyu, Wang Fang, Wang Binhua, Shi Xiao, Hou Qunhao, Huang Waigen, Lin Gang
Department of Bioscience, College of Life Science, Nanchang University, Nanchang 330031, China.
Department of Bioscience, College of Life Science, Nanchang University, Nanchang 330031, China.
Fish Shellfish Immunol. 2017 May;64:68-77. doi: 10.1016/j.fsi.2017.03.008. Epub 2017 Mar 6.
Grass carp (Ctenopharyngodon idella) was one of the economically important freshwater fish in China. However, hemorrhagic disease caused by grass carp reovirus (GCRV) results in a tremendous loss in the process of grass carp cultivation. Transcriptome analysis could provide a comprehensive understanding of the molecular mechanisms involved in specific biological processes and diseases for the resistance to reovirus infection of grass carp. In this study, the raw data from NCBI (accession number: SRA099702) were analyzed, in which, 50 significant differentially expressed genes by routine transcriptome analysis and 84 notably differentially expressed genes by co-expression network method. KEGG analysis revealed that the pathway in hemorrhagic diseases in grass carp was similar to the influenza A induced pathway. The interferon-stimulated gene ISG15 and sacsin-like gene, which were up-regulated in data (SRA099702), were also up-regulated in data (SRP049081) from a similar assay. QPCR experiment was performed to validate these up-regulated genes. The ISG15 gene was shown to be the core gene in the co-expression network. The results would enhance our understanding of the antivirus system of grass carp infected by reovirus.
草鱼（Ctenopharyngodon idella）是中国重要的经济淡水鱼之一。然而，草鱼呼肠孤病毒（GCRV）引起的出血性疾病在草鱼养殖过程中造成了巨大损失。转录组分析可以全面了解草鱼抗呼肠孤病毒感染的特定生物学过程和疾病所涉及的分子机制。在本研究中，对来自NCBI（登录号：SRA099702）的原始数据进行了分析，其中通过常规转录组分析鉴定出50个显著差异表达基因，通过共表达网络方法鉴定出84个显著差异表达基因。KEGG分析表明，草鱼出血性疾病的途径与甲型流感诱导的途径相似。在数据（SRA099702）中上调的干扰素刺激基因ISG15和类sacsin基因，在类似试验的数据（SRP049081）中也上调。进行了QPCR实验以验证这些上调基因。ISG15基因被证明是共表达网络中的核心基因。这些结果将增强我们对呼肠孤病毒感染草鱼的抗病毒系统的理解。
