Brito Ângela, Ramos Vitor, Mota Rita, Lima Steeve, Santos Arlete, Vieira Jorge, Vieira Cristina P, Kaštovský Jan, Vasconcelos Vitor M, Tamagnini Paula
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal.
Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007 Porto, Portugal; Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal.
Mol Phylogenet Evol. 2017 Jun;111:18-34. doi: 10.1016/j.ympev.2017.03.006. Epub 2017 Mar 6.
Aiming at increasing the knowledge on marine cyanobacteria from temperate regions, we previously isolated and characterized 60 strains from the Portuguese foreshore and evaluate their potential to produce secondary metabolites. About 15% of the obtained 16S rRNA gene sequences showed less than 97% similarity to sequences in the databases revealing novel biodiversity. Herein, seven of these strains were extensively characterized and their classification was re-evaluated. The present study led to the proposal of five new taxa, three genera (Geminobacterium, Lusitaniella, and Calenema) and two species (Hyella patelloides and Jaaginema litorale). Geminobacterium atlanticum LEGE 07459 is a chroococcalean that shares morphological characteristics with other unicellular cyanobacterial genera but has a distinct phylogenetic position and particular ultrastructural features. The description of the Pleurocapsales Hyella patelloides LEGE 07179 includes novel molecular data for members of this genus. The filamentous isolates of Lusitaniella coriacea - LEGE 07167, 07157 and 06111 - constitute a very distinct lineage, and seem to be ubiquitous on the Portuguese coast. Jaaginema litorale LEGE 07176 has distinct characteristics compared to their marine counterparts, and our analysis indicates that this genus is polyphyletic. The Synechococcales Calenema singularis possess wider trichomes than Leptolyngbya, and its phylogenetic position reinforces the establishment of this new genus.
为了增加对温带地区海洋蓝藻细菌的了解，我们之前从葡萄牙海岸分离并鉴定了60株菌株，并评估了它们产生次生代谢产物的潜力。所获得的16S rRNA基因序列中约15%与数据库中的序列相似度低于97%，这揭示了新的生物多样性。在此，对其中7株菌株进行了深入鉴定，并重新评估了它们的分类。本研究提出了五个新分类单元，即三个属（双杆菌属、卢西塔尼亚藻属和卡勒内马属）和两个种（盘状海链藻和滨海雅金藻）。大西洋双杆菌LEGE 07459是一种色球藻目蓝藻，与其他单细胞蓝藻属具有共同的形态特征，但具有独特的系统发育位置和特殊的超微结构特征。盘状海链藻LEGE 07179的描述包括了该属成员的新分子数据。卢西塔尼亚革藻的丝状分离株——LEGE 07167、07157和06111——构成了一个非常独特的谱系，似乎在葡萄牙海岸广泛存在。滨海雅金藻LEGE 07176与其海洋同类相比具有不同的特征，我们的分析表明该属是多系的。奇异卡勒内马的藻丝比细鞘丝藻更宽，其系统发育位置强化了这个新属的建立。
