Stern David L, Crocker Justin, Ding Yun, Frankel Nicolas, Kappes Gretchen, Kim Elizabeth, Kuzmickas Ryan, Lemire Andrew, Mast Joshua D, Picard Serge
Janelia Research Campus, Ashburn, Virginia 20147
Janelia Research Campus, Ashburn, Virginia 20147.
G3 (Bethesda). 2017 Apr 3;7(4):1339-1347. doi: 10.1534/g3.116.038885.
Species of the species subgroup, including the species , , , and , have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped transposons including an () gene expressed in the eyes and a ϕ site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in that will be useful for genetic mapping studies.
该物种亚组的物种，包括物种 、 、 和 ，长期以来一直作为研究进化的模型系统。然而，对这些物种的研究受到遗传和转基因试剂匮乏的限制。在这里，我们描述了为促进这些物种内部和之间的遗传研究而产生的一系列转基因和遗传菌株。我们已经为每个物种产生了许多含有定位转座子的菌株，包括在眼睛中表达的 （）基因和一个ϕ位点特异性整合位点。我们已经测试了这些品系的一个子集的整合效率和报告基因表达水平。我们还产生了一小批在眼睛中表达其他遗传编码荧光分子的其他品系以及一些其他转基因试剂，这些试剂将有助于这些物种的功能研究。此外，我们已经绘制了 中58个转座元件的插入位置，这将有助于遗传图谱研究。
