Mao Yuqiang, Han Yun, Shi Wenjun
Department of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
Onco Targets Ther. 2017 Feb 27;10:1217-1226. doi: 10.2147/OTT.S125742. eCollection 2017.
is a Ras-related maternally imprinted tumor suppressor gene. Loss of expression contributes to the malignant progression of various tumors. However, reports on the clinical implications and functional role of expression in esophageal squamous cell carcinoma (ESCC) are limited. This study examined the role of in ESCC.
In total, 81 patients diagnosed with ESCC based on histopathological evaluations who were subjected to surgical resection were included in the study. expression was analyzed by immunohistochemistry and western blotting, examining the correlations between expression and patient clinicopathological features. The functional effects of overexpression were examined using a Cell Counting Kit-8 assay, flow cytometry, a Transwell assay, wound healing, and western blotting in the ECA109 cell line.
was highly expressed in 27.5% (22/81) of ESCC specimens (adjacent noncancerous tissues, 85.2%, 69/81; <0.05). The expression level was significantly lower in patients with lymph node metastasis than in patients without (<0.05). A Kaplan-Meier survival analysis showed that patients with low expression had shorter survival than patients with high expression (<0.05), and a multivariate Cox analysis revealed that is an independent predictor of overall survival (=0.029). Finally, overexpression of in ESCC cells indicates that suppresses proliferative capacity, invasive capacity, and cell cycle progression and may also suppress epithelial-mesenchymal transition and induce apoptosis and autophagy.
may be a prognostic biomarker and a potential therapeutic target in ESCC.
[基因名称]是一种与Ras相关的母系印记肿瘤抑制基因。其表达缺失促进了各种肿瘤的恶性进展。然而，关于[基因名称]表达在食管鳞状细胞癌（ESCC）中的临床意义和功能作用的报道有限。本研究探讨了[基因名称]在ESCC中的作用。
本研究共纳入81例经组织病理学评估确诊为ESCC并接受手术切除的患者。通过免疫组织化学和蛋白质印迹法分析[基因名称]的表达，研究[基因名称]表达与患者临床病理特征之间的相关性。在ECA109细胞系中，使用细胞计数试剂盒-8检测、流式细胞术、Transwell检测、伤口愈合实验和蛋白质印迹法检测[基因名称]过表达的功能效应。
[基因名称]在27.5%（22/81）的ESCC标本中高表达（癌旁非癌组织中为85.2%，69/81；P<0.05）。有淋巴结转移的患者中[基因名称]表达水平显著低于无淋巴结转移的患者（P<0.05）。Kaplan-Meier生存分析显示，[基因名称]低表达患者的生存期短于高表达患者（P<0.05），多因素Cox分析显示[基因名称]是总生存期的独立预测因子（P=0.029）。最后，ESCC细胞中[基因名称]的过表达表明[基因名称]可抑制增殖能力、侵袭能力和细胞周期进程，还可能抑制上皮-间质转化并诱导凋亡和自噬。
[基因名称]可能是ESCC的预后生物标志物和潜在治疗靶点。
