Miśkowiak B, Stachowiak A, Zabel M, Malendowicz L K
Department of Histology and Embryology, Poznań Academy of Medicine, Poland.
Exp Clin Endocrinol. 1987 Sep;90(2):178-84. doi: 10.1055/s-0029-1210687.
The aim of the study was to investigate, by coupled immunocytochemical and stereologic methods, the effects of long-term intraventricular administration of ovine CRF (oCRF) on ACTH-immunoreactive cells of the rat anterior pituitary and to compare this effect with that of adrenalectomy. CRF-treated rats received 100 ng of oCRF daily for 8 days while control rats were administered with bovine serum albumin (BSA). After BSA administration number of ACTH-immunoreactive cells/mm2 was greater than in intact rats and CRF lowered their number, however, they were still more numerous than in intact rats. Neither average area nor average volume of studied cells were changed in control and CRF-treated animals if compared with intact ones. The total number of ACTH-immunoreactive cells was higher in the glands of BSA and CRF-treated rats than in intact animals. On the other hand, 60 h after bilateral adrenalectomy a marked increase in both, average area and average volume of ACTH-immunoreactive cells was found. The different response of rat corticotropes to intraventricular CRF administration and adrenalectomy may depend on the presence of operating negative corticosterone feedback in animals with adrenal glands intact. Moreover, the present study revealed the lack of correlation between the average area and average volume of ACTH-immunoreactive cells and the weight of the adrenal gland.
本研究旨在通过免疫细胞化学和体视学方法相结合，探讨长期脑室内注射羊促肾上腺皮质激素释放因子（oCRF）对大鼠垂体前叶促肾上腺皮质激素（ACTH）免疫反应性细胞的影响，并将其与肾上腺切除术的效果进行比较。经CRF处理的大鼠连续8天每天接受100 ng的oCRF，而对照大鼠则给予牛血清白蛋白（BSA）。给予BSA后，每平方毫米ACTH免疫反应性细胞的数量高于完整大鼠，CRF降低了其数量，但仍比完整大鼠多。与完整动物相比，对照和CRF处理动物中所研究细胞的平均面积和平均体积均未改变。BSA和CRF处理大鼠腺体中ACTH免疫反应性细胞的总数高于完整动物。另一方面，双侧肾上腺切除术后60小时，发现ACTH免疫反应性细胞的平均面积和平均体积均显著增加。大鼠促肾上腺皮质激素细胞对脑室内注射CRF和肾上腺切除术的不同反应可能取决于肾上腺完整动物中存在的负性皮质酮反馈作用。此外，本研究揭示了ACTH免疫反应性细胞的平均面积和平均体积与肾上腺重量之间缺乏相关性。
