Miśkowiak B, Leśniewska B, Nowak M, Malendowicz L K
Department of Histology and Embryology, Poznań Academy of Medicine, Poland.
Exp Clin Endocrinol. 1988 Sep;92(1):1-6. doi: 10.1055/s-0029-1210773.
The aim of the study was to investigate the effects of gonadectomy and sex hormones on responsiveness of anterior pituitary slices to synthetic CRF and on plasma ACTH level in male and female control, gonadectomised and testosterone or estradiol replaced rats. In animals of both sexes neither gonadectomy nor sex hormone replacement had an effect on plasma level of radioimmunoassayable ACTH. Adenohypophyseal ACTH output in vitro in response to CRF was unchanged by orchiectomy and lowered after testosterone replacement. Moreover, neither ovariectomy nor estradiol replacement had an effect on ACTH output by the entire adenohypophysis. Thus, since there were no apparent changes in plasma ACTH concentration among intact, gonadectomised and testosterone or estradiol replaced male and female rats as well as in responsiveness of the gland to CRF it seems that sex difference in the adrenocortical structure and function depends on action of sex hormones on adrenal and/or liver than on ACTH release on hypothalamic or pituitary level.
本研究旨在探讨性腺切除术和性激素对雄性和雌性对照、性腺切除及睾酮或雌二醇替代大鼠垂体前叶切片对合成促肾上腺皮质激素释放因子（CRF）的反应性以及血浆促肾上腺皮质激素（ACTH）水平的影响。在两性动物中，性腺切除术和性激素替代均未对放射免疫法可检测的血浆ACTH水平产生影响。睾丸切除术后，垂体前叶体外对CRF反应产生的促肾上腺皮质激素输出未发生变化，但在睾酮替代后降低。此外，卵巢切除术和雌二醇替代均未对整个垂体前叶的促肾上腺皮质激素输出产生影响。因此，由于完整、性腺切除及睾酮或雌二醇替代的雄性和雌性大鼠血浆ACTH浓度以及腺体对CRF的反应性均无明显变化，肾上腺皮质结构和功能的性别差异似乎取决于性激素对肾上腺和/或肝脏的作用，而非下丘脑或垂体水平上ACTH的释放。
