Plasmodium falciparum: mapping genes to nine parasite chromosomes.
作者信息
Weber J L
机构信息
Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
出版信息
Exp Parasitol. 1988 Feb;65(1):148-53. doi: 10.1016/0014-4894(88)90117-8.
PMID:2828102
Abstract
Using pulsed field gel electrophoresis with pulse time of 120 sec, eight chromosomal DNA molecules from clone 7G8 of the Plasmodium falciparum Brazilian isolate IMTM22 were resolved. A ninth chromosomal molecule which did not enter the gel was identified at the slot by hybridization to two DNA probes and by restriction enzyme analysis. Thirteen parasite DNA sequences were mapped to the nine chromosomes, with at least one sequence mapped to each chromosome. The restriction enzyme NotI appeared to produce only one cut in the entire IMTM22 genome.
摘要
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