Skorobogaty A, White R J, Phillips D R, Reiss J A
Department of Chemistry, La Trobe University, Bundoora, Victoria, Australia.
FEBS Lett. 1988 Jan 25;227(2):103-6. doi: 10.1016/0014-5793(88)80877-9.
The DNA-sequence specificity of daunomycin was investigated by DNase I footprinting and an E. coli RNA polymerase transcription-inhibition assay. The 5'-CA sequence was identified as being the highest affinity binding site, although other modest affinity (5'-GC, CG, CT, TC, AC) and poor affinity sites (5'-AA, AT, TA) were also observed. The preference of daunomycin for 5'-CA nucleotide sequence suggests that its biological activity may arise from association with the 5'-CA-containing sequences thought to be associated with genetic regulatory elements in eukaryotes.
通过DNA酶I足迹法和大肠杆菌RNA聚合酶转录抑制试验研究了柔红霉素的DNA序列特异性。5'-CA序列被确定为具有最高亲和力的结合位点，不过也观察到了其他中等亲和力（5'-GC、CG、CT、TC、AC）和低亲和力位点（5'-AA、AT、TA）。柔红霉素对5'-CA核苷酸序列的偏好表明，其生物活性可能源于与真核生物中被认为与基因调控元件相关的含5'-CA序列的结合。
