Vaquero Luis, Rodríguez-Martín Laura, Hernando Mercedes, Jiménez Marcos, Monteserín Luz, Alvarez-Cuenllas Begoña, Ruiz de Morales Jose M, Calleja Sara, Vivas Santiago
Department of Gastroenterology, University Hospital of León, León, Spain.
Department of Pathology, University Hospital of León, León, Spain.
Histol Histopathol. 2018 Jan;33(1):65-71. doi: 10.14670/HH-11-888. Epub 2017 Mar 10.
BACKGROUND/AIMS: In celiac disease there is an increase of lymphocytes expressing FOXP3 in the intestinal mucosa associated with varying degrees of villous atrophy. Our aim was to evaluate FOXP3 expression in duodenal mucosa with lymphocytic enteritis according to aetiology and correlation with lymphocytes T-γδ.
We compared three adult patient groups suffering lymphocytic enteritis: celiacs following a gluten-free diet (n=12), first-degree relatives of celiac patients with genetic risks (n=14) and patients with functional dyspepsia (n=14), along with a control group not suffering from duodenal enteritis (n=16). The population of duodenal lymphocytes was analysed by immunohistochemistry assays for CD3+ characterisation and FOXP3 expression. Quantification of lymphocytes T-γδ in duodenal mucosa was performed by flow cytometry in fresh tissue samples.
Presence of lymphocytes T-γδ was significantly higher in the group of celiac individuals compared to the group of relatives of these individuals (37.44 vs 5,52: p<0.0001) and the group with functional dyspepsia (37.44 vs 11.76: p=0.008). FOXP3 expression was also significantly higher in the celiac group than in the groups of relatives (18.85 vs 6.31; p=0.001) and functional dyspepsia patients (18.85 vs 7.61; p=0.023). The proportion of lymphocytes T-γδ and FOXP3- expressing lymphocytes was similar in the control group to that in the relatives or functional dyspepsia groups.
Lymphocytic enteritis associated to celiac disease shows an increase of FOXP3 expression and lymphocytes T-γδ that is not detected in other etiologies of enteritis.
背景/目的：在乳糜泻中，肠道黏膜中表达叉头框蛋白P3（FOXP3）的淋巴细胞增多，且伴有不同程度的绒毛萎缩。我们的目的是根据病因评估淋巴细胞性肠炎患者十二指肠黏膜中FOXP3的表达情况及其与T-γδ淋巴细胞的相关性。
我们比较了三组患有淋巴细胞性肠炎的成年患者：遵循无麸质饮食的乳糜泻患者（n = 12）、有遗传风险的乳糜泻患者一级亲属（n = 14）和功能性消化不良患者（n = 14），以及一组未患十二指肠肠炎的对照组（n = 16）。通过免疫组织化学分析对十二指肠淋巴细胞群体进行CD3 + 特征鉴定和FOXP3表达分析。通过流式细胞术对新鲜组织样本中的十二指肠黏膜T-γδ淋巴细胞进行定量分析。
与这些个体的亲属组（37.44对5.52：p < 0.0001）和功能性消化不良组（37.44对11.76：p = 0.008）相比，乳糜泻患者组中T-γδ淋巴细胞的数量显著更高。乳糜泻组中FOXP3的表达也显著高于亲属组（18.85对6.31；p = 0.001）和功能性消化不良患者组（18.85对7.61；p = 0.023）。对照组中T-γδ淋巴细胞和表达FOXP3的淋巴细胞比例与亲属组或功能性消化不良组相似。
与乳糜泻相关的淋巴细胞性肠炎显示FOXP3表达增加以及T-γδ淋巴细胞增多，这在其他肠炎病因中未被检测到。
