Huang J, Eng J, Yalow R S
Solomon A. Berson Research Laboratory, Veterans Administration Medical Center, Bronx, NY.
Horm Metab Res. 1987 Nov;19(11):542-4. doi: 10.1055/s-2007-1011878.
Glucagon is a 29 amino acid peptide that is generally highly conserved. Among mammalian glucagons the only one that has been shown to differ significantly is that of the guinea pig which differs from the others in 5 of the 9 COOH-terminus amino acids. The amino acid content and partial sequencing of chicken glucagon had been reported earlier. This report describes the purification and complete amino acid sequencing of chicken glucagon and demonstrates that it differs from the usual mammalian glucagon by the replacement of asparagine at position 28 with serine. Chicken glucagon is indistinguishable from porcine glucagon in the rat liver receptor assay system.
胰高血糖素是一种由29个氨基酸组成的肽，通常高度保守。在哺乳动物的胰高血糖素中，唯一被证明有显著差异的是豚鼠的胰高血糖素，它在9个羧基末端氨基酸中的5个与其他物种不同。鸡胰高血糖素的氨基酸含量和部分序列此前已有报道。本报告描述了鸡胰高血糖素的纯化和完整氨基酸序列，并证明它与通常的哺乳动物胰高血糖素不同，在第28位的天冬酰胺被丝氨酸取代。在大鼠肝脏受体检测系统中，鸡胰高血糖素与猪胰高血糖素无法区分。
