Péter Mária, Glatz Attila, Gudmann Péter, Gombos Imre, Török Zsolt, Horváth Ibolya, Vígh László, Balogh Gábor
Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
PLoS One. 2017 Mar 10;12(3):e0173739. doi: 10.1371/journal.pone.0173739. eCollection 2017.
Cell membranes actively participate in stress sensing and signalling. Here we present the first in-depth lipidomic analysis to characterize alterations in the fission yeast Schizosaccharomyces pombe in response to mild heat stress (HS). The lipidome was assessed by a simple one-step methanolic extraction. Genetic manipulations that altered triglyceride (TG) content in the absence or presence of HS gave rise to distinct lipidomic fingerprints for S. pombe. Cells unable to produce TG demonstrated long-lasting growth arrest and enhanced signalling lipid generation. Our results reveal that metabolic crosstalk between membrane and storage lipids facilitates homeostatic maintenance of the membrane physical/chemical state that resists negative effects on cell growth and viability in response to HS. We propose a novel stress adaptation mechanism in which heat-induced TG synthesis contributes to membrane rigidization by accommodating unsaturated fatty acids of structural lipids, enabling their replacement by newly synthesized saturated fatty acids.
细胞膜积极参与应激感知和信号传导。在此，我们展示了首次深入的脂质组学分析，以表征裂殖酵母粟酒裂殖酵母在轻度热应激（HS）下的变化。通过简单的一步甲醇提取法评估脂质组。在有无HS的情况下改变甘油三酯（TG）含量的基因操作产生了粟酒裂殖酵母独特的脂质组指纹图谱。无法产生TG的细胞表现出持久的生长停滞和信号脂质生成增强。我们的结果表明，膜脂与储存脂之间的代谢串扰有助于维持膜物理/化学状态的稳态，从而抵抗HS对细胞生长和活力的负面影响。我们提出了一种新的应激适应机制，其中热诱导的TG合成通过容纳结构脂质的不饱和脂肪酸来促进膜的刚性化，使其能够被新合成的饱和脂肪酸所取代。
