Henricks P A, Engels F, van der Vliet H, Nijkamp F P
Department of Pharmacology, State University of Utrecht, The Netherlands.
Int J Immunopharmacol. 1987;9(7):833-9. doi: 10.1016/0192-0561(87)90080-4.
Previously we showed that guinea pig alveolar macrophages (AMs) incubated with serum obtained from Haemophilus influenzae-treated animals had detrimental effects on airway smooth muscle beta-adrenergic receptor function. In the present study the influence of H. influenzae treatment on several functions of guinea pig AMs was examined. Sera obtained from animals 4 days after intraperitoneal administration of H. influenzae or from control guinea pigs possessed similar opsonic capacities. No effects of these sera on hydrogen peroxide release by AMs were observed as compared to the basal hydrogen peroxide release of AMs. Interestingly, stimulation of AMs with serum from control animals resulted in a diminished cyclo-oxygenase product formation, which was potentiated after incubating AMs with serum from H. influenzae-treated guinea pigs. No differences in phagocytic activity of AMs isolated from control or H. influenzae-treated animals were observed. When AMs were incubated with phorbol myristate acetate or zymosan, the cells produced superoxide anion and released hydrogen peroxide. However, the amounts of superoxide and hydrogen peroxide released did not differ between AMs isolated from control or H. influenzae-treated animals.
此前我们发现，用从经流感嗜血杆菌处理的动物体内获取的血清孵育豚鼠肺泡巨噬细胞（AMs），会对气道平滑肌β-肾上腺素能受体功能产生不利影响。在本研究中，我们检测了流感嗜血杆菌处理对豚鼠AMs若干功能的影响。在腹腔注射流感嗜血杆菌4天后从动物体内获取的血清，与从对照豚鼠体内获取的血清具有相似的调理能力。与AMs的基础过氧化氢释放量相比，未观察到这些血清对AMs过氧化氢释放有任何影响。有趣的是，用对照动物的血清刺激AMs会导致环氧化酶产物生成减少，而在用经流感嗜血杆菌处理的豚鼠的血清孵育AMs后，这种减少会增强。未观察到从对照动物或经流感嗜血杆菌处理的动物分离出的AMs在吞噬活性上存在差异。当AMs与佛波酯肉豆蔻酸酯或酵母聚糖一起孵育时，细胞会产生超氧阴离子并释放过氧化氢。然而，从对照动物或经流感嗜血杆菌处理的动物分离出的AMs释放的超氧和过氧化氢量并无差异。
