Li W, Bowen J M, Chan J C
Department of Tumor Biology, University of Texas, M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Intervirology. 1987;28(1):50-6. doi: 10.1159/000149996.
The 6M2 cell line was established by transformation of normal rat kidney cells with the ts110 mutant of Moloney murine sarcoma virus (Mo-MSV). P85gag-mos was found to be the only known viral-transforming protein in this cell system. Previously, we described the detection in 6M2 cells of rat-specific transformation-associated proteins (TAPs) using a monoclonal antibody (MC). In this study, we used MC to investigate further the expression of TAPs at different temperatures and the time course of turn-on and shut-off of TAPs upon temperature shifting. It was found that TAPs were expressed at 24, 28 and 33 degrees C, but not at 37 and 39 degrees C. In experiments of temperature shifting, TAPs reappeared in about 6 h in 6M2 cells after the temperature was shifted from 39 to 33 degrees C, following closely the reappearance of P85gag-mos. The data in this communication support the notion that TAPs might be activated by the v-mos gene product during the process of transformation.
6M2细胞系是通过用莫洛尼鼠肉瘤病毒（Mo-MSV）的ts110突变体转化正常大鼠肾细胞而建立的。P85gag-mos被发现是该细胞系统中唯一已知的病毒转化蛋白。此前，我们使用单克隆抗体（MC）描述了在6M2细胞中检测大鼠特异性转化相关蛋白（TAPs）的情况。在本研究中，我们使用MC进一步研究了TAPs在不同温度下的表达情况以及温度变化时TAPs开启和关闭的时间进程。结果发现，TAPs在24、28和33摄氏度时表达，但在37和39摄氏度时不表达。在温度变化实验中，当温度从39摄氏度转变为33摄氏度后，TAPs在6M2细胞中约6小时后重新出现，紧随P85gag-mos的重新出现。本通讯中的数据支持这样一种观点，即TAPs可能在转化过程中被v-mos基因产物激活。
