在ts110莫洛尼鼠肉瘤病毒转化的6M2细胞中，对不同于mos基因产物的转化相关蛋白（TAP）进行单克隆抗体检测。

Monoclonal antibody detection of transformation associated protein (TAP) in ts110 Moloney murine sarcoma virus transformed 6M2 cells that are different from the mos gene product.

作者信息

Chan J C, Keck M E, Li W

出版信息

Biochem Biophys Res Commun. 1986 Feb 13;134(3):1223-30. doi: 10.1016/0006-291x(86)90381-5.

Abstract

By the use of a highly specific monoclonal antibody (designated MC), we were able to detect three radiolabeled bands with molecular weights of 60,000, 63,000, and 66,000 daltons in the ts-110 Moloney murine sarcoma virus mutant-transformed rat kidney cells known as 6M2. Expression of transformation properties as well as these three bands in 6M2 cells was found to be temperature sensitive. Therefore, MC detected factors that are apparently associated with the transformation of 6M2 cells. These factors are tentatively referred to as transformation associated proteins. These transformation proteins were found in two other Moloney murine sarcoma virus-transformed rat cell lines. These proteins were found to differ from known gene products of the ts-110 Moloney murine sarcoma virus mutant and do not have kinase activity. The transformation associated proteins may represent rat cellular factors activated during the transformation of rat cells by Moloney murine sarcoma virus.

摘要

通过使用一种高度特异性的单克隆抗体（命名为MC），我们能够在被称为6M2的ts - 110莫洛尼鼠肉瘤病毒突变体转化的大鼠肾细胞中检测到三条放射性标记条带，其分子量分别为60,000、63,000和66,000道尔顿。发现在6M2细胞中转化特性以及这三条条带的表达对温度敏感。因此，MC检测到了明显与6M2细胞转化相关的因子。这些因子被暂时称为转化相关蛋白。在另外两种莫洛尼鼠肉瘤病毒转化的大鼠细胞系中也发现了这些转化蛋白。发现这些蛋白与ts - 110莫洛尼鼠肉瘤病毒突变体的已知基因产物不同，并且不具有激酶活性。转化相关蛋白可能代表在莫洛尼鼠肉瘤病毒转化大鼠细胞过程中被激活的大鼠细胞因子。