Koike K, Yoshida E, Katagiri K, Katayanagi M, Oda M, Tsunoo H, Yaginuma K, Kobayashi M
Department of Gene Research, Japanese Foundation for Cancer Research, Tokyo.
Jpn J Cancer Res. 1987 Dec;78(12):1341-6.
The human hepatoma cell line, huGK-14, containing integrated hepatitis B virus (HBV) DNAs, produced a large number of 23 nm spherical particles of HBV surface antigen (HBsAg) in a serum-free medium. These particles closely resembled the HBsAg particles found in the sera of HBV carriers and elicited a high antibody response in guinea pigs. The huGK-14 cells in a serum-free medium would provide a new, economic source of HBV vaccine.
人肝癌细胞系huGK - 14含有整合的乙型肝炎病毒（HBV）DNA，在无血清培养基中产生大量23纳米的HBV表面抗原（HBsAg）球形颗粒。这些颗粒与在HBV携带者血清中发现的HBsAg颗粒非常相似，并在豚鼠中引发了高抗体反应。无血清培养基中的huGK - 14细胞将提供一种新的、经济的HBV疫苗来源。
