Merten O W, Reiter S, Scheirer W, Katinger H
Dev Biol Stand. 1983;55:121-7.
The human cell line PLC/PRF/5 (5) was used for the production of hepatitis B surface antigen subtype ad (HBsAg ad) and purified by affinity chromatography (AC) with monoclonal antibodies (mAb). mAb to HBsAg from mouse ascites have been purified by Protein A - AC prior coupling to AH-Sepharose 4B (Pharmacia). The combined procedure of ammonium-sulphate-precipitation of HBsAg from culture supernatants and immunosorbent-AC leads to approx. 700-fold purification. ELISA results using the mAb and the HBsAg for diagnostics of human serum, positive for anti-HBsAg-antibodies correlate with the RIA (AUSAB, Abbott).
人细胞系PLC/PRF/5用于生产乙型肝炎表面抗原ad亚型（HBsAg ad），并通过与单克隆抗体（mAb）的亲和层析（AC）进行纯化。来自小鼠腹水的抗HBsAg单克隆抗体在与AH-琼脂糖4B（Pharmacia）偶联之前已通过蛋白A-AC进行了纯化。从培养上清液中硫酸铵沉淀HBsAg与免疫吸附-AC的联合程序可实现约700倍的纯化。使用该单克隆抗体和HBsAg诊断人血清的ELISA结果显示，抗HBsAg抗体呈阳性与放射免疫分析（AUSAB，雅培公司）结果相关。
