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新型蛋白质亲和反应简易快速平台。

New platform for simple and rapid protein-based affinity reactions.

机构信息

Graduate School of Engineering, Kyoto University, Kyoto, Japan.

Analytical and Quality Evaluation Research Laboratories, Daiichi Sankyo Co., Ltd., Hiratsuka, Japan.

出版信息

Sci Rep. 2017 Mar 14;7(1):178. doi: 10.1038/s41598-017-00264-y.

DOI:10.1038/s41598-017-00264-y
PMID:28282970
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5428043/
Abstract

We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.

摘要

我们开发了一种由聚（乙烯-co-甲基丙烯酸缩水甘油酯）组成的具有连续大孔的海绵状多孔聚合物（海绵状整体），允许环氧基团与感兴趣的蛋白质在原位进行有效反应。将蛋白 A 固定在海绵状整体上，即使在高流速下，也能从细胞培养上清液中高效收集免疫球蛋白 G（IgG）。此外，将胃蛋白酶固定在海绵状整体上，能够在高流速下进行有效的在线消化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/fe292dacabcb/41598_2017_264_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/c7b3660043d2/41598_2017_264_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/7978b006432f/41598_2017_264_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/255f38b712b9/41598_2017_264_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/1f7682f3471e/41598_2017_264_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/4fb5690b45bb/41598_2017_264_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/2b7d272ba3c1/41598_2017_264_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/d697a4ed7f75/41598_2017_264_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/fe292dacabcb/41598_2017_264_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/c7b3660043d2/41598_2017_264_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/7978b006432f/41598_2017_264_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/255f38b712b9/41598_2017_264_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/1f7682f3471e/41598_2017_264_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/4fb5690b45bb/41598_2017_264_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/2b7d272ba3c1/41598_2017_264_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/d697a4ed7f75/41598_2017_264_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40e2/5428043/fe292dacabcb/41598_2017_264_Fig8_HTML.jpg

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