Dorogova Natalia V, Bolobolova Elena Us, Zakharenko Lyudmila P
Institute of Cytology & Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Institute of Cytology & Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Dev Biol. 2017 Apr 15;424(2):105-112. doi: 10.1016/j.ydbio.2017.02.020. Epub 2017 Mar 7.
Gonadal atrophy is the most typical and dramatic manifestation of intraspecific hybrid dysgenesis syndrome leading to sterility in Drosophila melanogaster dysgenic progeny. The P-M system of hybrid dysgenesis is primarily associated with germ cell degeneration during the early stages of Drosophila embryonic development at elevated temperatures. In the present study, we have defined the phase of germ cell death as beginning at the end of embryogenesis immediately following gonad formation. However, the temperature-dependent screening of germ cell developmental patterns in the dysgenic background showed that early germ cells are susceptible to the hybrid dysgenesis at any Drosophila life-cycle stage, including in the imago. Electron microscopy of germ cells after dysgenesis induction revealed significant changes in subcellular structure, especially mitochondria, prior to cellular breakdown. The mitochondrial pathology can promote the activation of cell death pathways in dysgenic germ cells, which leads to gonadal atrophy.
性腺萎缩是种内杂交不育综合征最典型、最显著的表现，会导致黑腹果蝇不育后代出现不育。杂交不育的P-M系统主要与果蝇胚胎发育早期在高温下生殖细胞退化有关。在本研究中，我们将生殖细胞死亡阶段定义为在性腺形成后立即开始的胚胎发育末期。然而，在不育背景下对生殖细胞发育模式进行温度依赖性筛选表明，早期生殖细胞在果蝇生命周期的任何阶段，包括成虫期，都易受杂交不育的影响。诱导不育后对生殖细胞进行电子显微镜观察发现，在细胞解体之前，亚细胞结构，尤其是线粒体发生了显著变化。线粒体病变可促进不育生殖细胞中细胞死亡途径的激活，从而导致性腺萎缩。
