Department of Oncologic & Digestive Surgery, AP-HP, Hospital Lariboisière, 2 rue Ambroise Paré, 75475, Paris Cedex 10, France.
Université Paris Diderot, Sorbonne Paris Cité, CART, INSERM U965, 74575, Paris, France.
Surg Endosc. 2017 Oct;31(10):4034-4043. doi: 10.1007/s00464-017-5442-z. Epub 2017 Mar 10.
Detection of an incipient Peritoneal Carcinomatosis (PC) is still challenging, and there is a crucial need for technological improvements in order to diagnose and to treat early this condition. The aim of this study was to create a murine model of incipient PC and to explore the PC with Fujinon Intelligent Chromo Endoscopy (FICE) in order to determine the wavelengths of the white light (WL) spectre that offer the highest contrast between PC nodules and surrounding peritoneum.
Eighteen BALB/c mice had intraperitoneal injection of murine colonic cancer CT26 cells. Peritoneal exploration with FICE was performed at different times. For each PC nodule, 1 WL and 10 FICE images were recorded. Each image was then divided into its elementary red, green and blue band images. Depending on the FICE channel, each elementary image corresponds to a specific wavelength of the WL spectre. Through numerical analysis of these images, the value of the nodule and the background peritoneum were obtained, and the contrast value was calculated. Contrast values obtained with the different wavelengths were then compared.
PC grew in all the mice. The number as well as the size of PC nodules was increasingly high depending on the day of exploration. Mean PCI was 1.6 ± 1.2 at day 5, 7.7 ± 2.6 at day 8 and 15.0 ± 7.3 at day 10. A total number of 1805 elementary images of PC nodules were analysed. The wavelength that offered the best contrast between PC nodules and background peritoneum was 460 nm with a mean contrast value of 0.240 ± 0.151 (p < 0.0001).
This murine model of incipient PC is effective, reliable and reproducible. A monochromatic light with a wavelength at 460 nm offers the highest contrast between PC nodules and background peritoneum, allowing a better detection of PC.
检测早期腹膜癌（PC）仍然具有挑战性，因此迫切需要技术改进，以便早期诊断和治疗这种疾病。本研究旨在创建一个早期 PC 的小鼠模型，并使用 Fujinon Intelligent Chromo Endoscopy（FICE）探索 PC，以确定白光（WL）光谱中提供 PC 结节与周围腹膜之间最高对比度的波长。
18 只 BALB/c 小鼠接受了腹腔注射鼠结肠癌细胞 CT26。在不同时间用 FICE 进行腹膜探查。对于每个 PC 结节，记录 1 个 WL 和 10 个 FICE 图像。然后将每个图像分为其基本的红、绿和蓝波段图像。根据 FICE 通道，每个基本图像对应于 WL 光谱的特定波长。通过对这些图像进行数值分析，获得结节和背景腹膜的值，并计算对比度值。然后比较不同波长的对比度值。
PC 在所有小鼠中生长。PC 结节的数量和大小随着探查天数的增加而增加。第 5 天的平均 PCI 为 1.6±1.2，第 8 天为 7.7±2.6，第 10 天为 15.0±7.3。共分析了 1805 个 PC 结节的基本图像。PC 结节和背景腹膜之间对比度最佳的波长为 460nm，平均对比度值为 0.240±0.151（p<0.0001）。
这种早期 PC 的小鼠模型有效、可靠且可重复。波长为 460nm 的单色光提供了 PC 结节与背景腹膜之间的最高对比度，从而更好地检测 PC。
