通过基于硅胶的材料上的共价色谱法快速制备牛巯基白蛋白。
Rapid preparation of bovine mercaptalbumin by means of covalent chromatography on silica-based materials.
作者信息
Millot M C, Sebille B
机构信息
Laboratoire de Physico-Chimie des Biopolymères, Université Paris-Val-de-Marne, Creteil, France.
出版信息
J Chromatogr. 1987 Nov 6;408:263-73. doi: 10.1016/s0021-9673(01)81809-4.
PMID:2828393
Abstract
Modified polyvinylimidazole-coated silica materials containing disulphide groups were evaluated for the covalent chromatographic purification of bovine serum albumin. They are inert toward this protein and allow the isolation of 40 mg of mercaptalbumin (0.97-1.05 SH per mole) in less than 3 h. They are easily regenerated.
摘要
对含有二硫键的改性聚乙烯基咪唑包覆硅胶材料进行了用于牛血清白蛋白共价色谱纯化的评估。它们对这种蛋白质呈惰性，并且能够在不到3小时的时间内分离出40毫克的巯基白蛋白（每摩尔含0.97 - 1.05个巯基）。它们易于再生。
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