Taleb N L, Millot M C, Sebille B
Laboratoire de Physico-Chimie des Biopolymères, U.M.R 27, C.N.R.S, Université Paris XII, Thiais, France.
J Chromatogr A. 1997 Jul 25;776(1):45-53. doi: 10.1016/s0021-9673(97)00153-2.
Chiral chromatographic supports were obtained by continuously applying solutions contained HSA to ion-exchange columns. The columns were packed with silica modified with polyvinylimidazole and a copolymer polyvinylpyrrolidone-polyvinylimidazole (75:25) respectively, quaternized and crosslinked. Small changes in the concentration of NaCl during immobilization of HSA lead to variations in the amount of HSA bound to the supports. These variations have consequences in terms of chromatographic retention (k'), selectivity (alpha) and resolution (Rs) of enantiomers. The effects of varying the pH and organic modifier of the mobile phase on the chromatographic properties were also examined.
通过将含有HSA的溶液连续施加到离子交换柱上获得手性色谱载体。这些柱子分别填充有经聚乙烯基咪唑改性的硅胶和共聚物聚乙烯基吡咯烷酮-聚乙烯基咪唑（75:25），进行季铵化和交联处理。在固定化HSA过程中NaCl浓度的微小变化会导致与载体结合的HSA量发生变化。这些变化对手性异构体的色谱保留（k'）、选择性（α）和分离度（Rs）产生影响。还研究了流动相的pH值和有机改性剂变化对色谱性能的影响。
