Alvarado Roxana, Leiva Sergio
Instituto de Bioquímica & Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Casilla, 567, Valdivia, Chile.
Folia Microbiol (Praha). 2017 Sep;62(5):409-416. doi: 10.1007/s12223-017-0511-1. Epub 2017 Mar 10.
This study describes the taxonomic diversity of pigmented, agar-degrading bacteria isolated from the surface of macroalgae collected in King George Island, Antarctica. A total of 30 pigmented, agarolytic bacteria were isolated from the surface of the Antarctic macroalgae Adenocystis utricularis, Monostroma hariotii, Iridaea cordata, and Pantoneura plocamioides. Based on the 16S rRNA data, the agarolytic isolates were affiliated to the genera Algibacter, Arthrobacter, Brachybacterium, Cellulophaga, Citricoccus, Labedella, Microbacterium, Micrococcus, Salinibacterium, Sanguibacter, and Zobellia. Isolates phylogenetically related to Cellulophaga algicola showed the highest agarase activity in culture supernatants when tested at 4 and 37 °C. This is the first investigation of pigmented agar-degrading bacteria, members of microbial communities associated with Antarctic macroalgae, and the results suggest that they represent a potential source of cold-adapted agarases of possible biotechnological interest.
本研究描述了从南极乔治王岛采集的大型藻类表面分离出的产色素、降解琼脂细菌的分类多样性。从南极大型藻类囊腺藻、哈氏礁膜、心形海带和似蜈蚣藻的表面共分离出30株产色素、琼脂分解细菌。基于16S rRNA数据，琼脂分解菌株隶属于噬藻菌属、节杆菌属、短杆菌属、噬纤维菌属、柠檬酸球菌属、拉贝氏菌属、微杆菌属、微球菌属、盐杆菌属、血杆菌属和佐贝氏菌属。在4℃和37℃测试时，与嗜冷噬纤维菌亲缘关系相近的分离株在培养上清液中显示出最高的琼脂酶活性。这是对与南极大型藻类相关的微生物群落成员——产色素琼脂分解细菌的首次研究，结果表明它们可能是具有潜在生物技术价值的低温适应型琼脂酶的潜在来源。
