van der Hagen Eline A E, Fokkert Marion J, Kleefman Amanda M D, Thelen Marc H M, van den Berg Sjoerd A A, Slingerland Robbert J
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Clin Chem Lab Med. 2017 Aug 28;55(10):1530-1536. doi: 10.1515/cclm-2016-0944.
Measurement of adequate glucose concentrations is complicated by in vitro breakdown of glucose due to glycolysis. Unlike the commonly used NaF-EDTA and NaF-oxalate phlebotomy tubes, citrated NaF-EDTA tubes are reported to directly and thereby completely inhibit glycolysis. Recently, Greiner introduced the Vacuette® FC-Mix NaF-EDTA-citrate tube, currently the only NaF-citrate tube without volume-disturbing liquid additions available on the European market. Here we present its potential as alternative for the laborious and therefore unfeasible conditions for glucose sampling as recommended by the World Health Organization (WHO).
The FC-Mix tube was tested against the WHO recommended method of optimal laboratory conditions, both in healthy volunteers and pregnant woman undergoing oral glucose tolerance test (oGTT) for screening of gestational diabetes mellitus (GDM). Glucose concentrations were measured after different incubation times (0-48 h) and temperatures (room temperature, 37 °C), both in uncentrifuged whole blood and centrifuged material.
Deming regression analysis shows that glucose concentrations measured in the FC-Mix tube correlate to the WHO recommended method. Stability is maintained at room temperature for 48 h and at least 24 h at 37 °C. The use of the FC-Mix tube was also validated in screening for GDM and proved comparable to the WHO recommended method in diagnostic outcome.
The new Greiner FC-Mix tube combines the easy handling of a routine tube with dry additive with the ability to immediately inhibit glycolysis as in the WHO method for optimal pre-analytical and analytical conditions and performs equally to those conditions when screening for GDM.
由于糖酵解作用，体外葡萄糖分解会使葡萄糖浓度的准确测量变得复杂。与常用的氟化钠 - 乙二胺四乙酸（NaF - EDTA）和氟化钠 - 草酸盐采血管不同，据报道，枸橼酸钠 - NaF - EDTA采血管能直接并完全抑制糖酵解。最近，格赖纳公司推出了Vacuette® FC - Mix NaF - EDTA - 枸橼酸盐采血管，这是目前欧洲市场上唯一一款未添加影响体积液体的NaF - 枸橼酸盐采血管。在此，我们展示其作为世界卫生组织（WHO）推荐的用于葡萄糖采样的繁琐且不可行条件的替代方案的潜力。
在健康志愿者和接受口服葡萄糖耐量试验（oGTT）以筛查妊娠期糖尿病（GDM）的孕妇中，将FC - Mix采血管与WHO推荐的最佳实验室条件方法进行对比测试。在不同的孵育时间（0 - 48小时）和温度（室温、37°C）下，对未离心的全血和离心后的样本测量葡萄糖浓度。
德明回归分析表明，在FC - Mix采血管中测得的葡萄糖浓度与WHO推荐方法相关。在室温下可保持48小时的稳定性，在37°C下至少保持24小时。FC - Mix采血管在GDM筛查中的应用也得到了验证，并且在诊断结果上与WHO推荐方法相当。
新型格赖纳FC - Mix采血管将常规干添加剂采血管的便捷操作与立即抑制糖酵解的能力相结合，如同WHO方法中的最佳分析前和分析条件，在筛查GDM时表现与那些条件相当。
