Karlsson F A, Burman P, Lööf L, Mårdh S
Department of Internal Medicine, University Hospital, Uppsala, Sweden.
J Clin Invest. 1988 Feb;81(2):475-9. doi: 10.1172/JCI113344.
In autoimmune gastritis antibodies against a membrane-bound parietal cell antigen of previously unknown function are present in the sera of patients. In this study, a vesicular membrane preparation of porcine gastric mucosa cells was found to be a potent antigenic source. This preparation blocked greater than 90% of antibody binding to a lysate of gastric mucosa cells. The membrane fraction contained H+,K+-ATPase (EC 3.6.1.36) as the major protein, which in sodium dodecyl sulfate-polyacrylamide gel electrophoresis migrated with a weight of 92 kD. After reduction and alkylation, this component was resolved into two bands of similar staining intensity (92 and 88 kD). Immunoblotting analysis showed that sera of patients recognized antigen with pattern identical to the major protein of the vesicular membranes. Protein A-Sepharose beads preincubated with immunoglobulins of five individual patient (but not control) sera were all found to reduce both the H+,K+-ATPase activity and the amount of parietal cell antigen of a preparation of vesicular membranes solubilized in n-octylglucoside. Taken together, the results of this study indicate that the major parietal cell antigen is identical to the acid-producing enzyme, H+,K+-ATPase, of the parietal cell.
在自身免疫性胃炎患者的血清中存在针对一种功能此前未知的膜结合壁细胞抗原的抗体。在本研究中，发现猪胃黏膜细胞的囊泡膜制剂是一种有效的抗原来源。该制剂可阻断超过90%的抗体与胃黏膜细胞裂解物的结合。膜组分中含有H⁺，K⁺-ATP酶（EC 3.6.1.36）作为主要蛋白质，其在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中迁移时的分子量为92 kD。经过还原和烷基化后，该组分被分解为两条染色强度相似的条带（92 kD和88 kD）。免疫印迹分析表明，患者血清识别的抗原模式与囊泡膜的主要蛋白质相同。用五名个体患者（而非对照）血清的免疫球蛋白预孵育的蛋白A-琼脂糖珠均被发现可降低溶解于正辛基葡糖苷中的囊泡膜制剂的H⁺，K⁺-ATP酶活性和壁细胞抗原量。综上所述，本研究结果表明，主要的壁细胞抗原与壁细胞的产酸酶H⁺，K⁺-ATP酶相同。
