Mårdh S, Song Y H
Department of Medical and Physiological Chemistry, Biomedical Centre, Uppsala University, Sweden.
J Intern Med Suppl. 1990;732:77-82. doi: 10.1111/j.1365-2796.1990.tb01476.x.
The occurrence of auto-antibodies in patients with the autoimmune disease pernicious anaemia and in patients with active duodenal ulcers was investigated. In order to characterize antigenic structures, various cellular and subcellular fractions were prepared from pig gastric mucosa and from a homogenate of duodenal mucosa. By means of an enzyme-linked immunosorbent assay and immunoblotting, both the H+,K(+)-ATPase and pepsinogen/pepsin were shown to constitute the major antigens. All of the seven pernicious-anaemia sera that were tested contained auto-antibodies against both antigens, and the epitopes of the H+,K(+)-ATPase were shown to be localized on its cytoplasmic face. In 75% (18/24) of the sera from patients with duodenal ulcers, auto-antibodies were detected when using purified antigens. Six sera reacted with H+,K(+)-ATPase and twelve reacted with pepsinogen, one reacted with both antigens, and four sera reacted with the duodenal mucosal antigen. The occurrence of auto-antibodies indicates that there is a mucosal lesion and that immunological factors may be involved in the pathogenesis of the disease in some patients.
对自身免疫性疾病恶性贫血患者和活动性十二指肠溃疡患者体内自身抗体的出现情况进行了研究。为了鉴定抗原结构，从猪胃黏膜和十二指肠黏膜匀浆中制备了各种细胞和亚细胞组分。通过酶联免疫吸附测定法和免疫印迹法，结果表明H⁺，K⁺ -ATP酶和胃蛋白酶原/胃蛋白酶均构成主要抗原。所检测的7份恶性贫血血清均含有针对这两种抗原的自身抗体，且H⁺，K⁺ -ATP酶的表位定位于其胞质面。在十二指肠溃疡患者的血清中，75%（18/24）在使用纯化抗原时检测到自身抗体。6份血清与H⁺，K⁺ -ATP酶反应，12份与胃蛋白酶原反应，1份与两种抗原均反应，4份血清与十二指肠黏膜抗原反应。自身抗体的出现表明存在黏膜病变，且免疫因素可能在某些患者疾病的发病机制中起作用。
