Dpto. Sanidad Animal, Instituto De Ganadería De Montaña (CSIC-Universidad De León), Facultad De Veterinaria, Campus De Vegazana s/n, 24071, León, Spain.
Dpto. Sanidad Animal, Instituto De Ganadería De Montaña (CSIC-Universidad De León), Facultad De Veterinaria, Campus De Vegazana s/n, 24071, León, Spain.
Comp Immunol Microbiol Infect Dis. 2020 Apr;69:101422. doi: 10.1016/j.cimid.2020.101422. Epub 2020 Jan 17.
The local expression of WC1 γδ T lymphocytes subset has been evaluated by immunohistochemical methods at the different types of lesions present in cows naturally infected with Mycobacterium avium subsp. paratuberculosis (Map) and in non-infected control animals. Infected cattle were either in the latent/subclinical (focal lesions) or clinical (diffuse paucibacillary and multibacillary forms) stage of paratuberculosis. To assess the cell distribution, a differential cell count was carried out at the lamina propria, gut-associated lymphoid tissue and submucosa. A significant increase in the number of WC1 γδ T cells was observed in all the infected animals, regardless of the type of lesion. Cows with focal lesions showed higher number of labeled cells than those with diffuse forms, where no differences were found between the two types. This increase in the number of positively immunolabelled lymphocytes in infected animals was seen in the lamina propria, with higher values in those with focal lesions. While in the lymphoid tissue no differences in the numbers were observed, in animals with focal lesions, WC1 γδ T cells tended to be located at the periphery of the granulomas. These findings suggest a proinflammatory action of WC1 γδ T lymphocytes in bovine paratuberculosis, which might play an important role in the containment of the Map-infection in the focal granulomas located in the lymphoid tissue, helping to prevent the progression toward diffuse forms responsible for the clinical signs.
采用免疫组织化学方法评估了在天然感染牛分枝杆菌副结核亚种（Map）和未感染对照动物的不同类型病变中局部 WC1 γδ T 淋巴细胞亚群的表达。感染牛处于副结核病的潜伏/亚临床（局灶性病变）或临床（少菌性和多菌性弥漫性）阶段。为了评估细胞分布，在固有层、肠道相关淋巴组织和黏膜下层进行了差异细胞计数。所有感染动物的 WC1 γδ T 细胞数量均显著增加，无论病变类型如何。具有局灶性病变的奶牛显示出比具有弥漫性病变的奶牛更高数量的标记细胞，但在这两种类型之间未发现差异。在固有层中观察到感染动物中免疫标记阳性淋巴细胞数量增加，在具有局灶性病变的动物中，其值更高。虽然在淋巴组织中未观察到数量差异，但在具有局灶性病变的动物中，WC1 γδ T 细胞倾向于位于肉芽肿的外围。这些发现表明 WC1 γδ T 淋巴细胞在牛副结核病中具有促炎作用，这可能在控制位于淋巴组织中的局灶性肉芽肿中的 Map 感染方面发挥重要作用，有助于防止导致临床症状的弥漫性病变的进展。
