Nomura K, Imaizumi T, Sawamura D, Hashimoto I, Katabira Y
Department of Dermatology, Hirosaki University School of Medicine, Japan.
J Invest Dermatol. 1988 Feb;90(2):170-4. doi: 10.1111/1523-1747.ep12462164.
In order to investigate the role of inflammatory cells in altering the collagenase production by epidermolysis bullosa (EB) fibroblasts, macrophage and polymorphonuclear leukocyte (PMN) factors obtained from mouse peritoneal fluids were added to the fibroblast culture system, and collagenase activity was assayed after a 48-h incubation. Data obtained here revealed that the response of collagenase production by fibroblasts was quite different, depending on the type of EB. Namely, EB dystrophica recessiva (EBDR) (n = 2) fibroblasts produced significant amounts of collagenase in the range of 5.07 (U/ml) to 6.04 in response to macrophage-conditioned medium, macrophage lysate, and PMN lysate, compared with 0.13 in the absence of these. On the other hand, EB dystrophica dominans (EBDD) (n = 1) fibroblasts showed little or no overt increase in enzyme production in the presence of macrophage lysate and PMN lysate, which resulted in a moderate increase to 3.82 in response to macrophage-conditioned medium. Furthermore, EB simplex (EBS) (n = 1) fibroblasts produced collagenase up to 3.84 in response to these three factors. These factors can be inactivated by treating with trypsin, pronase, and phenylglyoxal. Our data clearly indicated that, in the comparisons of EBDD and EBS fibroblasts, EBDR fibroblasts showed quite high response to factors derived from macrophages and PMNs in terms of collagenase production. This fact may raise a clue that accounts for the high levels of tissue collagenase activity, which plays a potentially major role in blister formation in EBDR.
为了研究炎症细胞在改变大疱性表皮松解症（EB）成纤维细胞胶原酶产生中的作用，将从小鼠腹腔液中获得的巨噬细胞和多形核白细胞（PMN）因子添加到成纤维细胞培养系统中，并在孵育48小时后测定胶原酶活性。此处获得的数据显示，根据EB的类型，成纤维细胞胶原酶产生的反应有很大差异。具体而言，隐性营养不良型EB（EBDR）（n = 2）成纤维细胞在巨噬细胞条件培养基、巨噬细胞裂解物和PMN裂解物的作用下，产生的胶原酶量显著增加，范围在5.07（U/ml）至6.04之间，而在没有这些物质时为0.13。另一方面，显性营养不良型EB（EBDD）（n = 1）成纤维细胞在巨噬细胞裂解物和PMN裂解物存在时，酶产生几乎没有明显增加，而在巨噬细胞条件培养基作用下适度增加至3.82。此外，单纯型EB（EBS）（n = 1）成纤维细胞对这三种因子产生的胶原酶高达3.84。这些因子可用胰蛋白酶、链霉蛋白酶和苯乙二醛处理使其失活。我们的数据清楚地表明，在EBDD和EBS成纤维细胞的比较中，就胶原酶产生而言，EBDR成纤维细胞对来自巨噬细胞和PMN的因子反应相当高。这一事实可能为解释组织胶原酶活性水平升高提供线索，而组织胶原酶活性在EBDR的水疱形成中可能起主要作用。
