Adams Nicolaus G, Schwenke Piper, Smith G Jason, Trainer Vera L
Environmental and Fisheries Sciences Division, Northwest Fisheries Science Center, National Marine Fisheries Service, NOAA, 2725 Montlake Boulevard East, Seattle, Washington, 98112, USA.
Conservation Biology Division, Northwest Fisheries Science Center, National Marine Fisheries Service, NOAA, 2725 Montlake Boulevard East, Seattle, Washington, 98112, USA.
Protist. 2017 Apr;168(2):197-205. doi: 10.1016/j.protis.2017.01.002. Epub 2017 Jan 26.
Microsatellites are commonly used markers in population genetics and are increasingly being employed to determine population structure in phytoplankton populations. We have developed seven polymorphic microsatellite markers for the domoic-acid producing diatom Pseudo-nitzschia australis. Using these markers, thirty P. australis isolates were genotyped, 10 isolates were from Monterey Bay, California and 20 were from off the northern coast of Oregon. The number of alleles per locus ranged from two to eight and observed heterozygosities ranged from 0.11 to 0.70. All but two of the isolates were genetically distinct and initial population differentiation analysis indicated no significant differences between the Pacific Northwest isolates and the Monterey Bay isolates. Pseudo-nitzschia australis microsatellites appear to be species specific based on cross amplification tests with Pseudo-nitzschia fraudulenta (Cleve) Hasle, Pseudo-nitzschia seriata (Cleve) H.Peragallo, Pseudo-nitzschia pungens (Grunow ex Cleve) and Pseudo-nitzschia multiseries (Hasle) Hasle.
微卫星是群体遗传学中常用的标记，并且越来越多地被用于确定浮游植物群体的种群结构。我们为产软骨藻酸的硅藻澳大利亚伪菱形藻开发了七个多态性微卫星标记。利用这些标记，对30个澳大利亚伪菱形藻分离株进行了基因分型，其中10个分离株来自加利福尼亚州的蒙特雷湾，20个来自俄勒冈州北部海岸外。每个位点的等位基因数量从2到8不等，观察到的杂合度从0.11到0.70不等。除两个分离株外，所有分离株在基因上都是不同的，初步的种群分化分析表明西北太平洋分离株和蒙特雷湾分离株之间没有显著差异。基于与欺诈伪菱形藻（克利夫）哈斯勒、条纹伪菱形藻（克利夫）H.佩拉加洛、尖刺伪菱形藻（格鲁诺夫·埃克斯·克利夫）和多列伪菱形藻（哈斯勒）哈斯勒的交叉扩增试验，澳大利亚伪菱形藻微卫星似乎具有物种特异性。
