Hamada N, Imamura Y, Shingu M
Department of Virology, Kurume University School of Medicine, Japan.
J Med Virol. 1988 Jan;24(1):1-9. doi: 10.1002/jmv.1890240102.
Vaccine strain derivatives could be serially isolated from the feces of a healthy infant for about 30 days after the second administration of a trivalent poliovirus vaccine. These were all identified as poliovirus type 3, and no other types were detected. The titer in feces increased gradually until day 19, when it reached a maximum, after which it decreased. The plaque size altered roughly in parallel with the changes in the titer. On day 11 it began to increase, becoming the highest abruptly on day 15, and then fell again. Oligonucleotide mapping analysis of RNAs of isolated viruses showed that the samples from days 1 and 11 exhibited the same patterns as those shown by the vaccine strain RNA. However, on day 15 many of the preexisting spots had decreased, and new ones appeared. On day 27, the pattern was again similar to that of the original virus, rather than that of the day-15 virus. A nonmetric distance scaling and cluster analysis suggested that all the strains were derived from the same origin and that the polioviruses on days 1, 11, 21, and 27 had closer relationships, but the poliovirus on day 15 was different.
在第二次接种三价脊髓灰质炎病毒疫苗后，可从一名健康婴儿的粪便中连续分离出疫苗株衍生物约30天。这些均被鉴定为脊髓灰质炎3型，未检测到其他类型。粪便中的滴度逐渐升高，直至第19天达到最高值，之后下降。噬菌斑大小的变化与滴度变化大致平行。在第11天开始增大，在第15天突然达到最大，然后再次下降。对分离病毒的RNA进行寡核苷酸图谱分析表明，第1天和第11天的样本显示出与疫苗株RNA相同的模式。然而，在第15天，许多先前存在的斑点减少，新的斑点出现。在第27天，其模式再次与原始病毒相似，而非第15天病毒的模式。非度量距离缩放和聚类分析表明，所有毒株均源自同一来源，第1天、第11天、第21天和第27天的脊髓灰质炎病毒关系更密切，但第15天的脊髓灰质炎病毒不同。
