Hamada N, Tamada S, Shingu M
Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.
Arch Virol. 1990;111(1-2):63-74. doi: 10.1007/BF01310505.
Enteroviruses were isolated monthly for one year from feces in the intestine of 47 cattle. Judging from the isolation panel, it was suggested that endemic infection occurs. Genetic changes of isolated enteroviruses were traced using RNase T1 oligonucleotide fingerprint analysis and nonparametric distance scaling. Using some characteristics transitions of fingerprint patterns we could also trace some strains. These analyses suggested that in some strains drastic genetic changes may occur, which coincide with additional infections transmitted from other cows. Furthermore, it was indicated that the genetic changes of viruses isolated from cow R13 were not very drastic, but genetic changes were drastic for viruses isolated from cow R19. Overall, we could never observe the same fingerprint pattern using RNase T1. This study suggests that genetic changes tend to accumulate as time elapses, and at the same time, infection decreases.
在一年的时间里，每月从47头牛肠道内的粪便中分离肠道病毒。从分离结果来看，提示存在地方性感染。使用核糖核酸酶T1寡核苷酸指纹分析和非参数距离标度追踪分离出的肠道病毒的基因变化。利用指纹图谱的一些特征转变，我们也能够追踪一些毒株。这些分析表明，在一些毒株中可能发生了剧烈的基因变化，这与从其他奶牛传播来的额外感染相吻合。此外，结果表明从奶牛R13分离出的病毒的基因变化不是很剧烈，但从奶牛R19分离出的病毒的基因变化很剧烈。总体而言，使用核糖核酸酶T1我们从未观察到相同的指纹图谱。这项研究表明，随着时间的推移，基因变化倾向于积累，同时感染减少。
